Project:Growing bacteria: Difference between revisions
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====Requirements==== | ====Requirements==== | ||
* Easily cleaned + sterilised | * Easily cleaned + sterilised | ||
* Either a shaker powerful enough to agitate full beakers, or a magnetic stirrer (which we have already started building). | * Either a shaker powerful enough to agitate full beakers, or a magnetic stirrer (which we have already started building). Magnetic stirrer may lyse cells | ||
* Can be made dark inside | * Can be made dark inside | ||
* Adjustable temp | * Adjustable temp | ||
Revision as of 00:37, 15 March 2013
V. Fischeri
7th march
Two plates of v. fischeri brought to lab. They are bioluminescent and glow green in the dark. When we got them they had been growing for a few days on 'BOSS' media (BOSS medium: 1% peptone, 0.3% beef extract, 0.1% glycerol, 3% NaCl, 1.5% agar, pH 7.3)
9th march
Some cultures transferred to new plates. Protocol
13th march
Original cultures still glow, but nothing from the new ones
E. Coli
Equipment
We need to fix our incubator with shaker, or make a new one.
Requirements
- Easily cleaned + sterilised
- Either a shaker powerful enough to agitate full beakers, or a magnetic stirrer (which we have already started building). Magnetic stirrer may lyse cells
- Can be made dark inside
- Adjustable temp
- Low power if possible.
- Temperature mesaurement (simple thermometer would do)
- Does it need to be sealable?
