Make and run an agarose gel

Make gel

• Choose the gel concentration (higher the concentration the smaller the bands you can see). The following instructions are for a 1% of 50ml, which we normally use.
• Add 1 gram of agarose to 5g (or ml) of TBE 10x and 45g (or ml) of dH20.
• Mix and then microwave this until it is clear (normally around 1 min)
• Leave to cool, then add Ethidium Bromide stock solution at a concentration of 2ul/40ml (so about 2.25ul for this gel) at about 50°C.