PCR

From London Hackspace Wiki
Revision as of 10:02, 2 May 2015 by Mycoplasma (talk | contribs) (→‎Colony PCR)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigation Jump to search

Colony PCR

  • Take colony, mix with 500ul of RO water
  • Boiling lysis program
  • Then run PCR using Techne TC-312
  • M13 primers (LacZ region of PUC19) and S16 primers (universal bacteria)
  • COL program. 30 cycles. Annealing is 53C (ideal for S16, 56 would be better for M16). Hold at 4

PCR primer design

Retrieved from ‘https://wiki.london.hackspace.org.uk/w/index.php?title=PCR&oldid=46720’