Flow Cytometer - Revision history

Tolland at 15:08, 2 January 2015

2015-01-02T15:08:11Z

← Older revision		Revision as of 15:08, 2 January 2015
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	http://www.youtube.com/watch?v=y1waCEPpWPI		http://www.youtube.com/watch?v=y1waCEPpWPI

	[[Category::Biohacking]]		[[Category:Biohacking]]

Tolland at 15:03, 2 January 2015

2015-01-02T15:03:34Z

← Older revision		Revision as of 15:03, 2 January 2015
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	http://www.youtube.com/watch?v=y1waCEPpWPI		http://www.youtube.com/watch?v=y1waCEPpWPI

			[[Category::Biohacking]]

Tolland at 15:02, 2 January 2015

2015-01-02T15:02:39Z

← Older revision		Revision as of 15:02, 2 January 2015
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	~~{{EquipmentInfobox~~
	~~\| name = Flow Cytometer <!-- Name of the item. -->~~
	~~\| image = IMG 20130927 144739.jpg <!-- Image of the item. Leave with placeholder image if none exists. -->~~
	~~\| model = Coulter EPICS ALTRA <!-- Model -->~~
	~~\| category = Equipment <!-- Main category. Please leave alone to keep item in this category -->~~
	~~\| subcat = Biohacking<!-- Sub-category if one exists. Please check main listing to see other categories contained within the main one -->~~
	~~\| status = Scrapped<!-- Set to one of; Good working order, Faulty, Out of order, Under construction, Out of consumables, Scrapped, or Unknown -->~~
	~~\| consumables = Sheath Solution, Cleaning fluid, sample tubes<!-- Any items used up in normal operation, such as; ink, paper, saw-blades, cutting disks, oil, etc.. -->~~
	~~\| accessories = Unknown <!-- Any items associated with the equipment but not consumable, such as; drill bits, safety gloves, goggles, etc.. -->~~
	~~\| reqtraining = Yes<!-- Is training required to use this equipment? Yes or no. -->~~
	~~\| trainlink = Unknown <!-- If training is required, provide a link to training signup or contact page. Otherwise leave blank. -->~~
	~~\| acnode = <!-- Is the equipment ACnode locked? -->~~
	~~\| owner = LHS/biohackers <!-- Provide a link to owners members page if other than LHS -->~~
	~~\| origin = Donated by Imperial College London<!-- If via pledge, please link to the completed pledge page on the wiki -->~~
	~~\| location = In storage~~
	~~\| maintainers = tolland<!-- NO LINKS PLEASE; it currently breaks the template. If someone is nominated as managing the upkeep of this item, please list them here. -->~~
	~~\| template_ver = 1.1 <!-- Please do not change. Used for tracking out-of-date templates -->~~
	}}

	<s>Imperial College donated a Flow Cytometer. It was donated as working, but in need of repair. However there is a lot of damage due to corrosion, which might be impossible to fix for a reasonable amount of time and money.</s>		<s>Imperial College donated a Flow Cytometer. It was donated as working, but in need of repair. However there is a lot of damage due to corrosion, which might be impossible to fix for a reasonable amount of time and money.</s>

Tolland at 16:09, 2 December 2014

2014-12-02T16:09:13Z

← Older revision		Revision as of 16:09, 2 December 2014
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	\| category = Equipment <!-- Main category. Please leave alone to keep item in this category -->		\| category = Equipment <!-- Main category. Please leave alone to keep item in this category -->
	\| subcat = Biohacking<!-- Sub-category if one exists. Please check main listing to see other categories contained within the main one -->		\| subcat = Biohacking<!-- Sub-category if one exists. Please check main listing to see other categories contained within the main one -->
	\| status = ~~Faulty~~<!-- Set to one of; Good working order, Faulty, Out of order, Under construction, Out of consumables, Scrapped, or Unknown -->		\| status = Scrapped<!-- Set to one of; Good working order, Faulty, Out of order, Under construction, Out of consumables, Scrapped, or Unknown -->
	\| consumables = Sheath Solution, Cleaning fluid, sample tubes<!-- Any items used up in normal operation, such as; ink, paper, saw-blades, cutting disks, oil, etc.. -->		\| consumables = Sheath Solution, Cleaning fluid, sample tubes<!-- Any items used up in normal operation, such as; ink, paper, saw-blades, cutting disks, oil, etc.. -->
	\| accessories = Unknown <!-- Any items associated with the equipment but not consumable, such as; drill bits, safety gloves, goggles, etc.. -->		\| accessories = Unknown <!-- Any items associated with the equipment but not consumable, such as; drill bits, safety gloves, goggles, etc.. -->
Line 18:		Line 18:
	}}		}}

			<s>Imperial College donated a Flow Cytometer. It was donated as working, but in need of repair. However there is a lot of damage due to corrosion, which might be impossible to fix for a reasonable amount of time and money.</s>

	~~Imperial College donated a Flow Cytometer.~~ It ~~was donated as working~~, ~~but in need of repair~~. ~~However there~~ is ~~a lot of damage due~~ to ~~corrosion~~, ~~which might be impossible~~ to ~~fix for a reasonable amount~~ of ~~time and money~~.		<s>'''Status:''' It's sustained damage to the sampling area, and we are missing some bits. Repairing is likely to be very $$$, so we are planning to dismantle it and use the optics and lasers on other projects.</s>

			'''Status:''' The machine was too expensive to repair, and was too large to store and therefore has been scrapped. Several of the parts have been incorporated into other projects, or have been ebayed.

	The unit is too large to be stored in the biolab (1.8m X 0.8m = 1.44 square meters of floor space) and was previously moved to storage while we worked on it.		The unit is too large to be stored in the biolab (1.8m X 0.8m = 1.44 square meters of floor space) and was previously moved to storage while we worked on it.

	'''Status:''' It's sustained damage to the sampling area, and we are missing some bits. Repairing is likely to be very $$$, so we are planning to dismantle it and use the optics and lasers on other projects.

	There are a number of large external units some of which are for providing power and cooling to the large laser.		There are a number of large external units some of which are for providing power and cooling to the large laser.

Tolland at 15:42, 16 June 2014

2014-06-16T15:42:21Z

← Older revision		Revision as of 15:42, 16 June 2014
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	Imperial College donated a Flow Cytometer. ~~The unit~~ is ~~too large~~ to be ~~stored in the biolab (1~~.~~8m X 0.8m = 1.44 square meters of floor space)~~		Imperial College donated a Flow Cytometer. It was donated as working, but in need of repair. However there is a lot of damage due to corrosion, which might be impossible to fix for a reasonable amount of time and money.

	'''Status:''' We are ~~going~~ to dismantle it ~~for parts~~.		The unit is too large to be stored in the biolab (1.8m X 0.8m = 1.44 square meters of floor space) and was previously moved to storage while we worked on it.

			'''Status:''' It's sustained damage to the sampling area, and we are missing some bits. Repairing is likely to be very $$$, so we are planning to dismantle it and use the optics and lasers on other projects.

	There are a number of large external units some of which are for providing power and cooling to the large laser.		There are a number of large external units some of which are for providing power and cooling to the large laser.

	There is a good description of how a flow cytometer works here; http://media.invitrogen.com.edgesuite.net/tutorials/4Intro_Flow/player.html		There is a good description of how a flow cytometer works here; http://media.invitrogen.com.edgesuite.net/tutorials/4Intro_Flow/player.html

	~~It was donated as working, but in need of repair. There is quite a lot of damage due to corrosion, and to the sampling area which we are working on.~~

	The machine requires a dust free environment due to it's use of lasers and optics.		The machine requires a dust free environment due to it's use of lasers and optics.

Tolland at 15:31, 16 June 2014

2014-06-16T15:31:37Z

← Older revision		Revision as of 15:31, 16 June 2014
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	\| owner = LHS/biohackers <!-- Provide a link to owners members page if other than LHS -->		\| owner = LHS/biohackers <!-- Provide a link to owners members page if other than LHS -->
	\| origin = Donated by Imperial College London<!-- If via pledge, please link to the completed pledge page on the wiki -->		\| origin = Donated by Imperial College London<!-- If via pledge, please link to the completed pledge page on the wiki -->
	\| location = ~~Basement, PCB area/Darkroom<!-- Floor, room/zone and location within that area -->~~		\| location = In storage
	\| maintainers = tolland<!-- NO LINKS PLEASE; it currently breaks the template. If someone is nominated as managing the upkeep of this item, please list them here. -->		\| maintainers = tolland<!-- NO LINKS PLEASE; it currently breaks the template. If someone is nominated as managing the upkeep of this item, please list them here. -->
	\| template_ver = 1.1 <!-- Please do not change. Used for tracking out-of-date templates -->		\| template_ver = 1.1 <!-- Please do not change. Used for tracking out-of-date templates -->
Line 19:		Line 19:


	Imperial College donated a Flow Cytometer. The unit is ~~currently sitting on~~ 1.8m X 0.8m = 1.44 square meters of floor space ~~in the PCB area of the basement.~~		Imperial College donated a Flow Cytometer. The unit is too large to be stored in the biolab (1.8m X 0.8m = 1.44 square meters of floor space)

	'''Status:''' ~~Currently waiting~~ for ~~basement plumbing to complete, before proceeding with fixing the fluid-assembly at the front right of the instrument. It needs a dust cover, or some protection to access the laser cabinet~~.		'''Status:''' We are going to dismantle it for parts.

	There are a number of large external units some of which are for providing power and cooling to the large laser. ~~(I do not anticipate that the largest laser will be tested for some time, so I hope to move these into out-of-the-way storage, under the worktop in the biolab)~~		There are a number of large external units some of which are for providing power and cooling to the large laser.

	There is a good description of how a flow cytometer works here; http://media.invitrogen.com.edgesuite.net/tutorials/4Intro_Flow/player.html		There is a good description of how a flow cytometer works here; http://media.invitrogen.com.edgesuite.net/tutorials/4Intro_Flow/player.html

Czechton: Czechton moved page Equipment/Flow Cytometer to Flow Cytometer

2014-04-26T13:29:58Z

Czechton moved page Equipment/Flow Cytometer to Flow Cytometer

← Older revision

Revision as of 13:29, 26 April 2014

Czechton at 14:15, 25 April 2014

2014-04-25T14:15:32Z

← Older revision		Revision as of 14:15, 25 April 2014
Line 4:		Line 4:
	\| model = Coulter EPICS ALTRA <!-- Model -->		\| model = Coulter EPICS ALTRA <!-- Model -->
	\| category = Equipment <!-- Main category. Please leave alone to keep item in this category -->		\| category = Equipment <!-- Main category. Please leave alone to keep item in this category -->
	\| subcat = ~~Biology~~<!-- Sub-category if one exists. Please check main listing to see other categories contained within the main one -->		\| subcat = Biohacking<!-- Sub-category if one exists. Please check main listing to see other categories contained within the main one -->
	\| status = Faulty<!-- Set to one of; Good working order, Faulty, Out of order, Under construction, Out of consumables, Scrapped, or Unknown -->		\| status = Faulty<!-- Set to one of; Good working order, Faulty, Out of order, Under construction, Out of consumables, Scrapped, or Unknown -->
	\| consumables = Sheath Solution, Cleaning fluid, sample tubes<!-- Any items used up in normal operation, such as; ink, paper, saw-blades, cutting disks, oil, etc.. -->		\| consumables = Sheath Solution, Cleaning fluid, sample tubes<!-- Any items used up in normal operation, such as; ink, paper, saw-blades, cutting disks, oil, etc.. -->

Tolland at 10:35, 27 November 2013

2013-11-27T10:35:23Z

← Older revision		Revision as of 10:35, 27 November 2013
Line 25:		Line 25:
	There are a number of large external units some of which are for providing power and cooling to the large laser. (I do not anticipate that the largest laser will be tested for some time, so I hope to move these into out-of-the-way storage, under the worktop in the biolab)		There are a number of large external units some of which are for providing power and cooling to the large laser. (I do not anticipate that the largest laser will be tested for some time, so I hope to move these into out-of-the-way storage, under the worktop in the biolab)

	There is a good description of how a flow cytometer works here; http://~~probes~~.invitrogen.com~~/resources/education~~/tutorials/4Intro_Flow/player.html		There is a good description of how a flow cytometer works here; http://media.invitrogen.com.edgesuite.net/tutorials/4Intro_Flow/player.html

	It was donated as working, but in need of repair. There is quite a lot of damage due to corrosion, and to the sampling area which we are working on.		It was donated as working, but in need of repair. There is quite a lot of damage due to corrosion, and to the sampling area which we are working on.
Line 54:		Line 54:
	This is a AV presentation describing the basics of the technology;		This is a AV presentation describing the basics of the technology;

	http://~~probes~~.invitrogen.com~~/resources/education~~/tutorials/4Intro_Flow/player.html		http://media.invitrogen.com.edgesuite.net/tutorials/4Intro_Flow/player.html

Tolland: /* Educationz */

2013-10-13T15:19:03Z

Educationz

← Older revision		Revision as of 15:19, 13 October 2013
Line 41:		Line 41:

	Caltech course notes on microfluidics		Caltech course notes on microfluidics

	http://thesis.library.caltech.edu/4796/3/02_Chapter_2.pdf		http://thesis.library.caltech.edu/4796/3/02_Chapter_2.pdf


			Cornell book on microfluidics

			http://www.kirbyresearch.com/index.cfm/wrap/textbook/microfluidicsnanofluidics.html

← Older revision		Revision as of 15:02, 2 January 2015
Line 1:		Line 1:
	~~{{EquipmentInfobox~~
	~~\| name = Flow Cytometer <!-- Name of the item. -->~~
	~~\| image = IMG 20130927 144739.jpg <!-- Image of the item. Leave with placeholder image if none exists. -->~~
	~~\| model = Coulter EPICS ALTRA <!-- Model -->~~
	~~\| category = Equipment <!-- Main category. Please leave alone to keep item in this category -->~~
	~~\| subcat = Biohacking<!-- Sub-category if one exists. Please check main listing to see other categories contained within the main one -->~~
	~~\| status = Scrapped<!-- Set to one of; Good working order, Faulty, Out of order, Under construction, Out of consumables, Scrapped, or Unknown -->~~
	~~\| consumables = Sheath Solution, Cleaning fluid, sample tubes<!-- Any items used up in normal operation, such as; ink, paper, saw-blades, cutting disks, oil, etc.. -->~~
	~~\| accessories = Unknown <!-- Any items associated with the equipment but not consumable, such as; drill bits, safety gloves, goggles, etc.. -->~~
	~~\| reqtraining = Yes<!-- Is training required to use this equipment? Yes or no. -->~~
	~~\| trainlink = Unknown <!-- If training is required, provide a link to training signup or contact page. Otherwise leave blank. -->~~
	~~\| acnode = <!-- Is the equipment ACnode locked? -->~~
	~~\| owner = LHS/biohackers <!-- Provide a link to owners members page if other than LHS -->~~
	~~\| origin = Donated by Imperial College London<!-- If via pledge, please link to the completed pledge page on the wiki -->~~
	~~\| location = In storage~~
	~~\| maintainers = tolland<!-- NO LINKS PLEASE; it currently breaks the template. If someone is nominated as managing the upkeep of this item, please list them here. -->~~
	~~\| template_ver = 1.1 <!-- Please do not change. Used for tracking out-of-date templates -->~~
	}}

	<s>Imperial College donated a Flow Cytometer. It was donated as working, but in need of repair. However there is a lot of damage due to corrosion, which might be impossible to fix for a reasonable amount of time and money.</s>		<s>Imperial College donated a Flow Cytometer. It was donated as working, but in need of repair. However there is a lot of damage due to corrosion, which might be impossible to fix for a reasonable amount of time and money.</s>