LBL04006 - Revision history

Tolland: Replaced content with " The Biolab SOPs are now stored on github; https://github.com/london-biohackers/biohackspace-sops/blob/master/index.rst"

2015-10-13T20:13:47Z

Replaced content with " The Biolab SOPs are now stored on github; https://github.com/london-biohackers/biohackspace-sops/blob/master/index.rst"

← Older revision		Revision as of 20:13, 13 October 2015
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			The Biolab SOPs are now stored on github;
	~~== Using the Autoclave for Waste Deactivation ==~~		https://github.com/london-biohackers/biohackspace-sops/blob/master/index.rst
	~~{\| class="wikitable"~~
	~~\|Author: Tolland~~
	~~\|Approved by: S. Thompson~~
	~~\|SOP No. LBL04006~~
	\|-
	~~\|Signed:~~
	~~\|Signed:~~
	~~\|Effective from:~~
	\|-
	~~\|Date:~~
	~~\|Date:~~
	~~\|Last edited:~~
	\|}
	~~'''1. Purpose'''~~
	~~This describes the operation of the Melag EuroKlav 29V-S. The autoclave is used for three main procedures with respect to GMO deactivation:<br>~~
	~~i) sterilization of glassware and equipment for reuse~~
	~~ii) Deactivation of small volumes of liquid and solid media~~
	~~iii) Deactivation of large loads of liquid and solid media~~

	~~'''2. Scope'''~~

	~~This should be observed every time the autoclave is used within the LBL BioLab for GMO waste deactivation.~~

	~~'''3. Responsibilities'''~~

	The ~~operator of the autoclave is responsible for their own safety and that of others in the vicinity during its use. The particular risks presented in using this piece of equipment~~ are ~~primarily due to the high temperatures and steam released during evacuation.~~

	~~Upon removal of items from the autoclave be aware that they will likely still be hot, and care should be taken to check that glass or ceramic items are intact.~~

	~~'''4. Materials'''~~

	The autoclave requires a supply of '''RO water'''. If the water supply level falls below a minimum level, the operator will be required to top up the system. The RO water is available from the tank under the counter. The autoclave is filled into the tank from the top of the machine.

	~~The daily test, and waste deactivation runs of the autoclave require '''autoclave tape'''. If we are out of autoclave tape, please email the [mailti~~:~~hello@biohackspace.org BSO] and report that we are out of stock.~~


	~~'''5. Related documents'''~~

	~~...~~


	~~'''6. Definitions'''~~

	~~...~~


	~~'''7. Procedures'''~~

	~~7.1 Daily Test procedure~~

	~~Before using the autoclave for GMO deactivation each day, the operator should run a test strip through the autoclave to check that it is working to specification.~~

	~~The test strip should be stapled into the lab book, with a note indicating the operator and the date/time the test was run, along with any observations.~~

	~~7.1.1 Unwrapped equipment and glassware with no internal cavities can be sterilized using the Quick Programme (28 psi~~/~~134 C~~/~~3 mins)~~

	~~[[File:Screenshot-quick-programme.png]]~~

	7.~~1.2 Materials such as a solid and liquid media can be sterilized using the Universal Programme (28 psi/134 C~~/~~30 mins)~~

	~~[[File:Screenshot-universal-programme.png]]~~

	~~7.1.3 Large volumes of liquid or solid media should be processed with extra care.~~

	~~The autoclave should be preheated;~~

	~~[[File:Screenshot~~-~~preheat.png]]~~

	~~If possible, the media should be split into several autoclaves sessions. The load should be run under the Universal programme~~

	~~[[File:Screenshot-universal-programme.png]]~~



	~~7.2 Deactivation of liquid and solid GMO waste ==~~

	~~7.2.1 Transport the waste to the autoclave in a non-drip autoclavable tray~~
	~~7.2.2 Ensure there are no folds or containers which can trap air~~
	~~7.2.3 Sterilize the load using the Universal-Programme (2.1 bar~~/~~134 C/15 mins)~~
	~~7.2.4 once the programme has run, the waste should be transferred to the chemical kill bin, for final deactivation~~

	~~'''8. Resources'''~~
	- ~~Operating manual: http:/~~/~~www.eickemeyer.nl~~/~~images~~/~~products/2281/500032_Melag_Euroklav_23VS~~.~~pdf~~


	~~[[Category:Biohacking]]~~

Tolland: /* Using the Autoclave for Waste Deactivation */

2014-12-11T18:04:17Z

Using the Autoclave for Waste Deactivation

← Older revision		Revision as of 18:04, 11 December 2014
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	\|}		\|}
	'''1. Purpose'''		'''1. Purpose'''
	This describes the operation of the Melag EuroKlav 29V-S. The autoclave is used for ~~two~~ main procedures with respect to GMO deactivation:<br>		This describes the operation of the Melag EuroKlav 29V-S. The autoclave is used for three main procedures with respect to GMO deactivation:<br>
	i) sterilization of glassware and equipment for reuse		i) sterilization of glassware and equipment for reuse
	ii) Deactivation of small volumes of ~~GMO exposed~~ media~~, and GMO exposed waste and equipment.~~		ii) Deactivation of small volumes of liquid and solid media
	iii)		iii) Deactivation of large loads of liquid and solid media

	'''2. Scope'''		'''2. Scope'''

Tolland: /* Using the Autoclave for Waste Deactivation */

2014-12-11T18:02:48Z

Using the Autoclave for Waste Deactivation

← Older revision		Revision as of 18:02, 11 December 2014
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	\|}		\|}
	'''1. Purpose'''		'''1. Purpose'''
	This describes the operation of the Melag EuroKlav 29V-S. The autoclave is used for two main procedures:<br>		This describes the operation of the Melag EuroKlav 29V-S. The autoclave is used for two main procedures with respect to GMO deactivation:<br>
	i) sterilization of ~~media,~~ glassware and ~~autoclavable~~ equipment ~~prior to another protocol~~		i) sterilization of glassware and equipment for reuse
	ii) Deactivation of ~~GMOs~~, and GMO exposed waste and equipment.		ii) Deactivation of small volumes of GMO exposed media, and GMO exposed waste and equipment.
			iii)

	'''2. Scope'''		'''2. Scope'''
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	7.1 Daily Test procedure		7.1 Daily Test procedure

	Before using the autoclave for GMO deactivation, the operator should run a test strip through the autoclave to check that it is working to specification.		Before using the autoclave for GMO deactivation each day, the operator should run a test strip through the autoclave to check that it is working to specification.

	The test strip should be stapled into the lab book, with a note indicating the operator and the date/time the test was run, along with any observations.		The test strip should be stapled into the lab book, with a note indicating the operator and the date/time the test was run, along with any observations.

	7.1.1 ~~Sterilize~~ the ~~load~~ using the Universal Programme (28 psi/134 C/30 mins)		7.1.1 Unwrapped equipment and glassware with no internal cavities can be sterilized using the Quick Programme (28 psi/134 C/3 mins)

			[[File:Screenshot-quick-programme.png]]

			7.1.2 Materials such as a solid and liquid media can be sterilized using the Universal Programme (28 psi/134 C/30 mins)

			[[File:Screenshot-universal-programme.png]]

			7.1.3 Large volumes of liquid or solid media should be processed with extra care.

			The autoclave should be preheated;

			[[File:Screenshot-preheat.png]]

			If possible, the media should be split into several autoclaves sessions. The load should be run under the Universal programme

			[[File:Screenshot-universal-programme.png]]

Tolland at 11:32, 27 November 2014

2014-11-27T11:32:17Z

← Older revision		Revision as of 11:32, 27 November 2014
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	'''4. Materials'''		'''4. Materials'''

	The autoclave requires a supply of RO water. If the water supply level falls below a minimum level, the operator will be required to top up the system. The RO water is available from the tank under the counter. The autoclave is filled into the tank from the top of the machine.		The autoclave requires a supply of '''RO water'''. If the water supply level falls below a minimum level, the operator will be required to top up the system. The RO water is available from the tank under the counter. The autoclave is filled into the tank from the top of the machine.

	The daily test, and waste deactivation runs of the autoclave require autoclave tape. If we are out of autoclave tape, please email the [mailti:hello@biohackspace.org BSO] and report that we are out of stock.		The daily test, and waste deactivation runs of the autoclave require '''autoclave tape'''. If we are out of autoclave tape, please email the [mailti:hello@biohackspace.org BSO] and report that we are out of stock.


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	The test strip should be stapled into the lab book, with a note indicating the operator and the date/time the test was run, along with any observations.		The test strip should be stapled into the lab book, with a note indicating the operator and the date/time the test was run, along with any observations.

	7.1.1 Use the * Sterilize the load using the ~~Quick-~~Programme (15 psi/~~121~~ C/15 mins)		7.1.1 Sterilize the load using the Universal Programme (28 psi/134 C/30 mins)

Tolland: /* Using the Autoclave for Waste Deactivation */

2014-11-27T11:28:40Z

Using the Autoclave for Waste Deactivation

← Older revision		Revision as of 11:28, 27 November 2014
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	The autoclave requires a supply of RO water. If the water supply level falls below a minimum level, the operator will be required to top up the system. The RO water is available from the tank under the counter. The autoclave is filled into the tank from the top of the machine.		The autoclave requires a supply of RO water. If the water supply level falls below a minimum level, the operator will be required to top up the system. The RO water is available from the tank under the counter. The autoclave is filled into the tank from the top of the machine.

			The daily test, and waste deactivation runs of the autoclave require autoclave tape. If we are out of autoclave tape, please email the [mailti:hello@biohackspace.org BSO] and report that we are out of stock.

Tolland: /* Using the Autoclave for Waste Deactivation */

2014-11-27T11:26:24Z

Using the Autoclave for Waste Deactivation

← Older revision		Revision as of 11:26, 27 November 2014
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	'''4. Materials'''		'''4. Materials'''

	The autoclave requires a supply of RO water. If the water supply level falls below a minimum level, the operator will be required to top up the system.		The autoclave requires a supply of RO water. If the water supply level falls below a minimum level, the operator will be required to top up the system. The RO water is available from the tank under the counter. The autoclave is filled into the tank from the top of the machine.

Tolland: /* Using the Autoclave for Waste Deactivation */

2014-11-27T08:46:45Z

Using the Autoclave for Waste Deactivation

← Older revision		Revision as of 08:46, 27 November 2014
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	'''7. Procedures'''		'''7. Procedures'''

	7.1 ~~Sterilization of liquid media~~		7.1 Daily Test procedure

			Before using the autoclave for GMO deactivation, the operator should run a test strip through the autoclave to check that it is working to specification.

			The test strip should be stapled into the lab book, with a note indicating the operator and the date/time the test was run, along with any observations.

	7.1.1 Use the * Sterilize the load using the Quick-Programme (15 psi/121 C/15 mins)		7.1.1 Use the * Sterilize the load using the Quick-Programme (15 psi/121 C/15 mins)

Tolland: /* Using the Autoclave for Waste Deactivation */

2014-11-27T08:42:48Z

Using the Autoclave for Waste Deactivation

← Older revision		Revision as of 08:42, 27 November 2014
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	'''2. Scope'''		'''2. Scope'''

	This should be observed every time the autoclave is used within the LBL BioLab.		This should be observed every time the autoclave is used within the LBL BioLab for GMO waste deactivation.

	'''3. Responsibilities'''		'''3. Responsibilities'''

	The operator of the autoclave is responsible for their own safety and that of others in the vicinity during its use. The particular risks presented in using this piece of equipment are primarily due to the high temperatures ~~generated~~, ~~steam~~ and ~~high pressures~~.		The operator of the autoclave is responsible for their own safety and that of others in the vicinity during its use. The particular risks presented in using this piece of equipment are primarily due to the high temperatures and steam released during evacuation.

			Upon removal of items from the autoclave be aware that they will likely still be hot, and care should be taken to check that glass or ceramic items are intact.

	'''4. Materials'''		'''4. Materials'''

	...		The autoclave requires a supply of RO water. If the water supply level falls below a minimum level, the operator will be required to top up the system.

Tolland: Created page with " == Using the Autoclave for Waste Deactivation == {| class="wikitable" |Author: Tolland |Approved by: S. Thompson |SOP No. LBL04006 |- |Signed: |Signed: |Effective from: |- |..."

2014-11-27T08:29:46Z

Created page with " == Using the Autoclave for Waste Deactivation == {| class="wikitable" |Author: Tolland |Approved by: S. Thompson |SOP No. LBL04006 |- |Signed: |Signed: |Effective from: |- |..."

New page

== Using the Autoclave for Waste Deactivation ==
{| class="wikitable"
|Author: Tolland
|Approved by: S. Thompson
|SOP No. LBL04006
|-
|Signed:
|Signed:
|Effective from:
|-
|Date:
|Date:
|Last edited:
|}
'''1. Purpose'''
This describes the operation of the Melag EuroKlav 29V-S. The autoclave is used for two main procedures:<br>
i) sterilization of media, glassware and autoclavable equipment prior to another protocol
ii) Deactivation of GMOs, and GMO exposed waste and equipment.

'''2. Scope'''

This should be observed every time the autoclave is used within the LBL BioLab.

'''3. Responsibilities'''

The operator of the autoclave is responsible for their own safety and that of others in the vicinity during its use. The particular risks presented in using this piece of equipment are primarily due to the high temperatures generated, steam and high pressures.

'''4. Materials'''

...

'''5. Related documents'''

...

'''6. Definitions'''

...

'''7. Procedures'''

7.1 Sterilization of liquid media

7.1.1 Use the * Sterilize the load using the Quick-Programme (15 psi/121 C/15 mins)

7.2 Deactivation of liquid and solid GMO waste ==

7.2.1 Transport the waste to the autoclave in a non-drip autoclavable tray
7.2.2 Ensure there are no folds or containers which can trap air
7.2.3 Sterilize the load using the Universal-Programme (2.1 bar/134 C/15 mins)
7.2.4 once the programme has run, the waste should be transferred to the chemical kill bin, for final deactivation

'''8. Resources'''
- Operating manual: http://www.eickemeyer.nl/images/products/2281/500032_Melag_Euroklav_23VS.pdf

[[Category:Biohacking]]