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Project:Plant species testing: Difference between revisions
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→Repeat without PCR (1/8/12):
Mycoplasma (talk | contribs) |
Mycoplasma (talk | contribs) |
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*5.3 grams of crushed pea added to 20ml of distilled, sterile water and a shake of salt. Solid removed and 3ml of detergent added | *5.3 grams of crushed pea added to 20ml of distilled, sterile water and a shake of salt. Solid removed and 3ml of detergent added | ||
* Shake of meat tenderiser added to break down proteins | * Shake of meat tenderiser added to break down proteins | ||
* Centrifuged and supernatant removed - solids discarded. | |||
* 5ml of 70% added to 10ml of template for precipitation | |||
* Interface removed and as much liquid as possible removed from this | |||
* Pellet dried at 60°C in thermocycler. | |||
(in each ladder tube there is 14 ul) | (in each ladder tube there is 14 ul) | ||
