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Project:OD600 Measurement: Difference between revisions
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At the moment the main issue is that the LDR (light dependent resistor) only sees about 20 ohms of difference between the initial inoculation and the mid-log phase, which shows as 0.4 OD600 on a spectrophotometer. | At the moment the main issue is that the LDR (light dependent resistor) only sees about 20 ohms of difference between the initial inoculation and the mid-log phase, which shows as 0.4 OD600 on a spectrophotometer. | ||
If you don't have access to a spectrophotometer, (or even if you do), this should be a convenient way to estimate OD600 values for your sample | |||
A secondary project is to cast some 10mm x 10mm resin blocks to be used for calibration against a system that takes a cuvette. | |||
Here is a document describing various methods for calibrating spectrophotometers; | |||
http://www.perkinelmer.co.uk/CMSResources/Images/44-136839TCH_Validating_UV_Visible.pdf | |||
