179
edits
Electrophoresis Stains: Difference between revisions
From London Hackspace Wiki
Added a few more dyes
No edit summary |
(Added a few more dyes) |
||
| Line 62: | Line 62: | ||
=== Acridine Orange === | === Acridine Orange === | ||
* Info: http://www.sigmaaldrich.com/catalog/product/sigma/a6014?lang=en®ion=GB | |||
* Interestingly enough, it glows orange-red when bound to single-stranded nucleic acids, and green when bound to double-strnaded nucleic acids. | |||
* Why it's not viable: Mutagen (though it may be worth trying, for the double/single strand differentiation alone) | |||
=== Propidium Iodide === | === Propidium Iodide === | ||
* Info: http://www.sigmaaldrich.com/catalog/product/sigma/p4170?lang=en®ion=GB | |||
* Why it's not viable: apparently not commonly used as a stain for PCR visualisation. Also, it's a mutagen. | |||
=== GelStar === | === GelStar === | ||
* Info: http://www.lonza.com/products-services/bio-research/nucleic-acid-and-protein-electrophoresis/nucleic-acid-electrophoresis/nucleic-acid-gel-stains/gelstar-nucleic-acid-gel-stain-10000x.aspx?WT.srch=1&gclid=CPfSoq-QzLECFQRTfAodDCAAbQ | |||
* Why it's not viable: Mutagen. | |||
=== EvaGreen === | === EvaGreen === | ||
* Info: http://www.bioscience.co.uk/product~88421 | |||
* Cost of product: £175 for 5ml, 2.5ul per sample. £0.0875 per sample | |||
* Total cost: For four samples, £0.35 | |||
* Why it's not viable: qPCR only, which we're not doing yet. | |||
== Discussion == | == Discussion == | ||
Here is a Web page with more information and roughly similar costings to mine: http://bitesizebio.com/articles/ethidium-bromide-the-alternatives/ | Here is a Web page with more information and roughly similar costings to mine: http://bitesizebio.com/articles/ethidium-bromide-the-alternatives/ | ||
Here is a Word doc with information on various dyes and disposal methods, along with indications of which ones are mutagenic: http://www.facilities.rochester.edu/cu/ElectrophoresisBufferSolutionsandGelDisposalGuidelines.doc | |||
Three comments on all this: | Three comments on all this: | ||
