Project:OD600 Measurement: Difference between revisions

From London Hackspace Wiki

m
Line 49: Line 49:


The idea is to make a cheap, robust, reliable alternative to a dedicated and expensive device. The initial brain storming is to take a 600 nm source, (or a broad wavelength source of light which includes 600 nm and use a BP filter) and a level detector, and calibrate it to match 0.4 at OD600 on a proper spectrophotometer..
The idea is to make a cheap, robust, reliable alternative to a dedicated and expensive device. The initial brain storming is to take a 600 nm source, (or a broad wavelength source of light which includes 600 nm and use a BP filter) and a level detector, and calibrate it to match 0.4 at OD600 on a proper spectrophotometer..
[[File:OD600-proto-1.png|thumb]]


The initial attempt was to use a white led with a filter for something approximating 600nm
The initial attempt was to use a white led with a filter for something approximating 600nm
Line 70: Line 72:


http://www.perkinelmer.co.uk/CMSResources/Images/44-136839TCH_Validating_UV_Visible.pdf
http://www.perkinelmer.co.uk/CMSResources/Images/44-136839TCH_Validating_UV_Visible.pdf


== alternative ideas ==
== alternative ideas ==