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Project:OD600 Measurement: Difference between revisions
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At the moment the main issue is that the LDR (light dependent resistor) only sees about 20 ohms of difference between the initial inoculation and the mid-log phase, which shows as 0.4 OD600 on a spectrophotometer. | At the moment the main issue is that the LDR (light dependent resistor) only sees about 20 ohms of difference between the initial inoculation and the mid-log phase, which shows as 0.4 OD600 on a spectrophotometer. | ||
It really needs some amplification and a lot more light shielding. | |||
If you don't have access to a spectrophotometer, (or even if you do), this should be a convenient way to estimate OD600 values for your sample | If you don't have access to a spectrophotometer, (or even if you do), this should be a convenient way to estimate OD600 values for your sample | ||
