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Project:OD600 Measurement: Difference between revisions
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In the molecular biology of bacteria, OD600 is often used to estimate the current growth phase of a bacteria or other cells in suspension. It means the "optical density of the sample to light of 600nm". The measured OD600 of the sample relative to a plain reference sample, gives an estimate of the concentration of the cells in the liquid, which can be used to estimate the growth phase of the population | In the molecular biology of bacteria, OD600 is often used to estimate the current growth phase of a bacteria or other cells in suspension. It means the "optical density of the sample to light of 600nm". The measured OD600 of the sample relative to a plain reference sample, gives an estimate of the concentration of the cells in the liquid, which can be used to estimate the growth phase of the population. | ||
The basic idea is to take a sample of plain media, and a sample of media in which bacteria has been growing. Initially the levels of bacteria will be very low, and the amount of light absorbed by the 2 samples will be similar. | The basic idea is to take a sample of plain media, and a sample of media in which bacteria has been growing. Initially the levels of bacteria will be very low, and the amount of light absorbed by the 2 samples will be similar. | ||
[[File:OD600-pictore.png|500px]] | [[File:OD600-pictore.png|500px]] [[File:14853028140 6a23c3757f k.jpg|300px]] | ||
Over time these will change according to which | |||
[[File:Growthcurve.jpeg|300px]] | [[File:Growthcurve.jpeg|300px]] | ||
600nm is in the visible wavelength an is approximately orange. | |||
