Difference between revisions of "BioLab Manual"
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| Line 10: | Line 10: | ||
* [[Make and run an agarose gel]] | * [[Make and run an agarose gel]] | ||
* Growing Escherichia coli | * Growing Escherichia coli | ||
| + | * Growing Vibrio fischeri ([[Project: Growing bacteria|our first attempt]]) | ||
== Untested == | == Untested == | ||
Revision as of 12:02, 25 April 2014
Safety
Protocols
Established
The protocols we have got working in the Biohackspace:
- DNA extraction using Chelex 100
- DNA extraction and precipitation with ethanol / isopropanol
- Make and run an agarose gel
- Growing Escherichia coli
- Growing Vibrio fischeri (our first attempt)
Untested
The protocols we we would like to get working in the Biohackspace.
Media
Equipment
- Biobot
- Thermal Cycler
- Electrophoresis Power Supply
- Big centrifuge
- Small Centrifuge
- Gel Electrophoresis Box (How we made our own)
- Scales & Measures
- Autoclave
- Incubator
- Gilson Pipettes
- Microscope
- Various Consumables
Strains
Reagents
The reagents we currently have stocked:
- A little bit of Monica in my life
- A little bit of Erica by my side
- A little bit of Rita is all I need
- A little bit of Tina is what I see
- A little bit of Sandra in the sun
- A little bit of Mary all night long
- A little bit of Jessica here I am
- A little bit of you makes me your man
See also Project:Making our own reagents