Revision as of 18:59, 9 January 2015

Safety

All users of the Biolab must follow the Biolab Usage Guidelines for their own and other user's safety.

For more extensive databases of protocols:

The protocols we have got working in the Biohackspace:

The protocols we would like to get working in the Biohackspace.

To create a page for a piece of lab equipment fill out this form and make sure you put "Biohacking" as the subcategory.

We also have an array of glassware and consumables.

This site has a directory of service engineers for various biomedical/biochemical equipment:

The reagents we currently have stocked

General Reagents
Reagent	Brand	Location
Bacto Agar		Shelves
Bacto Yeast Extract		Shelves
LB broth powder		Shelves
TBE buffer x10		Shelves
Sodium Acetate		Shelves
Ethidium Bromide		Drawers
Tri-Sodium Citrate-2 Water		Shelves
Magnesium Chloride		Shelves
Calcium Chloride		Shelves
Agarose		Shelves
Glycerin		Shelves
Ampicillin Sodium Salt (powdered)		Fridge
Kanamycin Sulphate (powdered)		Fridge
IPTG		Freezer
Proteinase K 1mg/ml		Freezer

DNA (Primers, Plasmids, etc.)
Reagent	Description	Brand
pUC19 control DNA	NEB #N3041A	NEB
Q5 Hi-GC enhancer	5x concentrate	NEB

Restriction Enzymes
Reagent	Cutting Site	Brand

   link to shopping list G spreadsheet

Sigma-Aldrich - Good for custom primers
Cole-Parmer
Farnell
Chang bioscience - pipettes
NBSBio - Good for ladders and cheap agarose (only low concentration) NB: Be sure to navigate to http://www.nbsbio.co.uk NOT http://nbsbio.co.uk, or their payment system doesn't work. Weird. Standard shipping cost is £15
Web scientific - Taq and ladder
New England Biolabs They sell very cheap taq. We could also look at their restriction enzymes. However atm they won't sell to us as they only sell to universities or registered biolabs. See this post and this post. Once we have all our registrations for LBL sorted we could try again.
Thermoscientific - Recommended for good value restriction enzymes. Not tried yet.
VWR - Big supplier of chemicals, glassware and various lab stuff. Sounds very positive about selling to the hackspace.
Invitrogen We failed their due diligence check. See post here and here. They have cheap TBE.
IDT - possible source for primers. Haven't tried them yet.
cheaplabstuff (ebay) - Cheap 1.5ml and 15ml tubes in small quantities. Also pasteur pipettes.
microsupplies (ebay) - Cheap 5-200ul and 100-1000ul tips by the 1000
Cambridge Bioscience - Not yet used, may supply GelRed or GelGreen (enquiry by Bene)

Also see general hackspace suppliers page.

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 = Safety =
-Please be aware that the substances you are working with maybe highly toxic. Consult [[Substance and Toxicity databases]] before if you are unfamiliar with the compounds with which you will be working.
-=== No mouth pipetting ===
+All users of the Biolab must follow the [[Biolab Usage Guidelines]] for their own and other user's safety.
-This is fairly self explanatory, and is also specifically mentioned in the regulations. (I presume due to some fairly horrific accidents)
-=== No unlabelled containers ===
-Unlabelled containers are a hazard, even if they are not hazardous.
-It makes it difficult to keep the lab tidy if there are random concoctions cluttering the shelves.
-Please label with the following;
-* nick
-* date
-* contents
-and if you are going to be leaving something hazardous or plan to leave an experiment running for an extended duration, please mail the list.
 = Protocols =