Difference between revisions of "BioLab Manual"
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= Safety =
= Safety =
Revision as of 20:06, 26 January 2015
All users of the Biolab must follow the BioLab Usage Guidelines for their own and other user's safety.
Instructions for making general stock solutions
For more extensive databases of protocols:
The protocols we have got working in the Biohackspace:
- DNA extraction using Chelex 100
- DNA extraction and precipitation with ethanol / isopropanol
- Make and run an agarose gel
- Solid Media Bacterial Culture
- Liquid Media Bacterial Culture
- Anachem Miniprep (plasmid purification)
The protocols we would like to get working in the Biohackspace.
To create a page for a piece of lab equipment fill out this form and make sure you put "Biohacking" as the subcategory.
We also have an array of glassware and consumables.
This site has a directory of service engineers for various biomedical/biochemical equipment:
The reagents we currently have stocked
|Bacto Yeast Extract||Shelves|
|LB broth powder||Shelves|
|TBE buffer x10||Shelves|
|Tri-Sodium Citrate-2 Water||Shelves|
|Ampicillin Sodium Salt (powdered)||Fridge|
|Kanamycin Sulphate (powdered)||Fridge|
|Proteinase K 1mg/ml||Freezer|
|pUC19 control DNA||NEB #N3041A||NEB|
|Q5 Hi-GC enhancer||5x concentrate||NEB|
See also: Project:Making our own reagents
link to shopping list G spreadsheet
- Sigma-Aldrich - Good for custom primers
- Chang bioscience - pipettes
- NBSBio - Good for ladders and cheap agarose (only low concentration) NB: Be sure to navigate to http://www.nbsbio.co.uk NOT http://nbsbio.co.uk, or their payment system doesn't work. Weird. Standard shipping cost is £15
- Web scientific - Taq and ladder
- New England Biolabs They sell very cheap taq. We could also look at their restriction enzymes. However atm they won't sell to us as they only sell to universities or registered biolabs. See this post and this post. Once we have all our registrations for LBL sorted we could try again.
- Thermoscientific - Recommended for good value restriction enzymes. Not tried yet.
- VWR - Big supplier of chemicals, glassware and various lab stuff. Sounds very positive about selling to the hackspace.
- Invitrogen We failed their due diligence check. See post here and here. They have cheap TBE.
- IDT - possible source for primers. Haven't tried them yet.
- cheaplabstuff (ebay) - Cheap 1.5ml and 15ml tubes in small quantities. Also pasteur pipettes.
- microsupplies (ebay) - Cheap 5-200ul and 100-1000ul tips by the 1000
- Cambridge Bioscience - Not yet used, may supply GelRed or GelGreen (enquiry by Bene)
Also see general hackspace suppliers page.