Difference between revisions of "Group:Biohacking"
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| − | == Biohacking | + | {{Group |
| + | |logo=Biohack_logo.png | ||
| + | |name=Biohacking | ||
| + | |when=Every Wednesday | ||
| + | |what= | ||
| + | |time=19:30 | ||
| + | |recurring=1}} | ||
| − | We' | + | We are the '''biohacking''' or '''DIY biology''' ([http://openwetware.org/wiki/DIYbio/FAQ Official FAQ about DIY-Biology]) group at the London Hackspace, a mix of amateur and professional biologists, attracted by the potential of molecular and synthetic biology. Anyone is welcome, so get involved! |
| − | + | Over the past couple of years we've been developing our equipment, optimising techniques and running some research projects. Here's quite a good short [http://www.bbc.co.uk/news/uk-england-london-19964886 local news video] showing our (previous, smaller) lab and some of what we do in it. | |
| − | |||
| − | We also | + | We will soon be meeting every month, check back here for our 2019 restart. |
| + | We have a list of [[Biohacking FAQ|frequently-asked questions]]. | ||
| + | |||
| + | [[:Category:Biohacking|'''List of biohacker wiki pages.''']] | ||
| + | |||
| + | We also maintain an outward facing website at [http://biohackspace.org/ BioHackspace.org] | ||
| + | |||
| + | == How to find us == | ||
| + | # Email us [mailto:hello@biohackspace.org here]. You can also sign up to our mailing list where we do most of our communication [https://lists.kentgeek.org/mailman/listinfo/lhs-biohacking here] (if you see a warning ignore it) (Your posts will be moderated unless you're signed up to the list. If you want to be signed up to the list but not receive messages you can change this in the options). You can view the mailing list archives through the previous link or through the [https://groups.google.com/forum/?fromgroups#!forum/london-biohackers google groups] interface. To chat in more detail request a signup to our slack channel using the email address above, or join our irc channel at [http://webchat.freenode.net/?channels=london-biohackers #london-biohackers on freenode] | ||
| + | |||
| + | == Biohackspace Membership == | ||
| + | We're a subgroup of the London Hackspace, and our rent and utility bills are paid as part of the larger space. Therefore it's London Biohackers also need to be members of the hackspace. You can find out how to become a hackspace member [https://london.hackspace.org.uk/signup.php here]. | ||
| + | |||
| + | There is a separate subscription for the biohacking group. Biohacking is more expensive than the typical hackspace activity, and with your membership we can pay for reagents, lab consumables and any equipment we may need for our experiments. The suggested minimum donation is £2 a week, but again, you can come along without joining. If you start to come regularly and use consumables then we'd like you to pay. Details on how to setup payment to become a biohackspace member are here: [[London Biohackers Membership | Details of Membership]]. | ||
| + | |||
| + | We also have an arrangement for people who just want to support the biohackspace work and lab costs, and do not intend to regularly attend the biolab to do science. If you want to become a remote member, or to donate equipment and make a regular contribution, and you can make contact [mailto:hello@biohackspace.org here by email], or see the [[Remote Membership|Donations/Remote members]] page for more details. | ||
| + | |||
| + | However it's not essential to become a member just to come along initially and find out what we do. | ||
| + | |||
| + | == Organization == | ||
| + | |||
| + | The biohackspace operates as a Company Limited by guarantee. The legal details of this are [[London Biological Laboratories|here]] | ||
| + | |||
| + | == Projects == | ||
| + | We are currently well equipped to carry out molecular biology experiments. The lab space on Hackney Road was a CL1 approved lab and we are aiming to reestablish that in Wembley. | ||
| + | |||
| + | === Current Projects === | ||
| + | * Hacking the lab | ||
| + | * * Recommissioning RO water system | ||
| + | * * Recommissioning Laminar Flow system | ||
| + | * * Building Laminar Flow System into a hood | ||
| + | * Culturing Local Algae | ||
| + | * Lab Automation | ||
| + | * Stirling Engine for Cryogenics | ||
| + | |||
| + | === Completed Projects === | ||
| + | |||
| + | * [http://2015.igem.org/Team:London_Biohackspace iGEM 2015] | ||
| + | * [[Project:iGEM 2014]] | ||
| + | * [[Project:Flow Hood]] | ||
| + | * [[E. coli transformation]] | ||
| + | * [[Image Focus Stacking]] | ||
| + | * [[CL1_BSO_SOP_GLP|Lab Containment Level 1]] | ||
| + | * [[Project:Growing bacteria|Growing V. fischeri]] | ||
| + | * [[Project:Sex typing with amelogenin]] | ||
| + | * [[Project:Public Biobrick]] (in collaboration with the UCL igem team) | ||
| + | * [[Blood typing]] | ||
| + | * [[Project:Plant species testing]] | ||
| + | * [[Project:Gel electrophoresis box]] | ||
| + | * [[Project:Shaker platform]] | ||
| + | |||
| + | === Future Projects === | ||
| + | * [[Project:Making our own reagents]] | ||
| + | *[[MindControlBacteria]] | ||
| + | * More and more becoming current project :) | ||
| + | Add your own! | ||
| + | |||
| + | == Our lab == | ||
| + | Our lab is small in size but we have tried to make good use of the tight space to incorporate all the equipment we need to carry out interesting projects. You can see a [[Lab|floor plan of the lab here]]. | ||
| + | |||
| + | We <strike> are currently working towards being </strike> are a certified Containment Level 1 Lab ([[BioSafety|more details on biosafety classifications]]). | ||
| + | |||
| + | The Lab is the registered office of our limited company, [[London Biological Laboratories]], used to deal with suppliers. | ||
| + | |||
| + | === How to use the Lab === | ||
| + | Please do not touch things in the lab if you do not know what they are or how to use them. Many items may be hazards to your health if used incorrectly. | ||
| + | |||
| + | Our [[BioLab Manual|lab manual]] details our equipment, reagents, strains and our established protocols. | ||
| + | |||
| + | === Starting your own lab? === | ||
| + | We have some notes on [[Starting your own biolab|starting your own biolab]] based on our experiences. | ||
| + | |||
| + | === Equipment we made === | ||
| + | Our hope is to be able to use self-developed or open source hardware equipment where possible in order to carry out our projects. Here is what we have done so far: | ||
| + | |||
| + | * [[Project:Gel electrophoresis box]] | ||
| + | * [[Biobot]] | ||
| + | * [[Project:Shaker platform]] | ||
| + | |||
| + | A list of biohacking hardware projects is here [[Project:Biohardware]] | ||
| + | |||
| + | === Equipment Pledges === | ||
| + | |||
| + | [[Biohacking Wishlist]] | ||
| + | |||
| + | * [[Pledge: Flow Hood]] | ||
| + | * [[Pledge:Lab Microscope]] | ||
| + | * [[Pledge:vacum forming machine]] | ||
| + | * [[Pledge:Gluconacetobacter_hansenii]] | ||
== Resources == | == Resources == | ||
| Line 12: | Line 105: | ||
*[http://www.nature.com/nature/comics/syntheticbiologycomic/ Nature.com comic about Synthetic Biology] - Nice introduction to playing with genetic engineering/cloning, though aimed at kids | *[http://www.nature.com/nature/comics/syntheticbiologycomic/ Nature.com comic about Synthetic Biology] - Nice introduction to playing with genetic engineering/cloning, though aimed at kids | ||
| − | *[http://www.youtube.com/watch?v=9W5ZshwteUw Bugs' Crash Course in Molecular Biology] - Recorded at the hackspace | + | *[http://www.youtube.com/watch?v=9W5ZshwteUw Bugs' Crash Course in Molecular Biology] - Recorded at the hackspace. [http://wiki.london.hackspace.org.uk/view/File:Intro_to_BioHacking.pdf Slides] |
*[http://www.youtube.com/watch?v=muxDfkKDXnY Sara's Introduction the the Biohacking Community] - Recorded at the hackspace | *[http://www.youtube.com/watch?v=muxDfkKDXnY Sara's Introduction the the Biohacking Community] - Recorded at the hackspace | ||
| − | + | [http://diyhpl.us/~bryan/papers2/bio/books/Primer%20for%20Synthetic%20Biology.pdf A primer for synthetic biology] | |
*[http://learn.genetics.utah.edu/content/labs/gel/ Good introduction to gel electrophoresis] - Explaining one of the core techniques for working with DNA | *[http://learn.genetics.utah.edu/content/labs/gel/ Good introduction to gel electrophoresis] - Explaining one of the core techniques for working with DNA | ||
*[http://learn.genetics.utah.edu/content/labs/pcr/ Good introduction to the Polymerase Chain Reaction (PCR)] - Explaining one of the core techniques for working with DNA | *[http://learn.genetics.utah.edu/content/labs/pcr/ Good introduction to the Polymerase Chain Reaction (PCR)] - Explaining one of the core techniques for working with DNA | ||
*[http://www.nature.com/news/2010/101006/full/467650a.html Nature.com article on Biohacking] - Free access. Nice explanation of what's needed in a lab and how different groups are managing it. | *[http://www.nature.com/news/2010/101006/full/467650a.html Nature.com article on Biohacking] - Free access. Nice explanation of what's needed in a lab and how different groups are managing it. | ||
| + | *[[Bioinformatics_resources | Bioinformatics Resources]] | ||
| + | *[http://wiki.london.hackspace.org.uk/w/images/4/43/Biohacking_workshop_Aug2012.pdf Slides] from Bioinformatics workshop August 2012 | ||
| + | *[http://en.wikipedia.org/wiki/User:Cathalgarvey/Books/A_Beginner's_Guide_to_Biotechnology Cathal Garvey's beginner's guide to biotech] | ||
| + | *[https://docs.google.com/drawings/d/1hXO4uu_TZ_yI3amJ1jGo9zu2XU3gX9yXMchC9gGZT6k/edit Flowchart diagram of DNA extraction protocol] | ||
| − | |||
=== Community === | === Community === | ||
*[http://diybio.org/blog Interesting DIYBio community blog] - Interesting blog taking articles from a few different groups | *[http://diybio.org/blog Interesting DIYBio community blog] - Interesting blog taking articles from a few different groups | ||
| − | *[http://biocurious.org/index.php?title=Main_Page Bio Curious] - A well-established | + | *[http://biocurious.org/index.php?title=Main_Page Bio Curious] - A well-established biohacking group in California |
*[http://www.tmplab.org/wiki/index.php/Brain_Computer_Interface Brain-Computer interface at Paris hackspace] | *[http://www.tmplab.org/wiki/index.php/Brain_Computer_Interface Brain-Computer interface at Paris hackspace] | ||
*[http://www.nature.com/nbt/journal/v27/n12/full/nbt1209-1077.html Nature.com article about biohacking community] - Not the same one as above. Interesting, but behind a paywall :(. | *[http://www.nature.com/nbt/journal/v27/n12/full/nbt1209-1077.html Nature.com article about biohacking community] - Not the same one as above. Interesting, but behind a paywall :(. | ||
** you can access it here: [http://www.synbioproject.org/process/assets/files/6452/_draft/nbt-2009-12d_-_biotech_nin_the_basement.pdf http://www.synbioproject.org/process/assets/files/6452/_draft/nbt-2009-12d_-_biotech_nin_the_basement.pdf] -- [http://heybryan.org/ kanzure] [[Special:Contributions/70.114.205.110|70.114.205.110]] 19:40, 2 January 2011 (UTC) | ** you can access it here: [http://www.synbioproject.org/process/assets/files/6452/_draft/nbt-2009-12d_-_biotech_nin_the_basement.pdf http://www.synbioproject.org/process/assets/files/6452/_draft/nbt-2009-12d_-_biotech_nin_the_basement.pdf] -- [http://heybryan.org/ kanzure] [[Special:Contributions/70.114.205.110|70.114.205.110]] 19:40, 2 January 2011 (UTC) | ||
| − | == | + | == Proposed code of conduct == |
| − | + | See here: http://wiki.hackspace.org.uk/wiki/Biohacking/Code_of_Conduct | |
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| − | + | [[Category:Biohacking]] | |
| − | + | [[Category:Groups]] | |
Latest revision as of 06:16, 1 May 2019
| Biohacking | |
|---|---|
| |
| When | Every Wednesday, from 19:30 |
| Members | |
We are the biohacking or DIY biology (Official FAQ about DIY-Biology) group at the London Hackspace, a mix of amateur and professional biologists, attracted by the potential of molecular and synthetic biology. Anyone is welcome, so get involved!
Over the past couple of years we've been developing our equipment, optimising techniques and running some research projects. Here's quite a good short local news video showing our (previous, smaller) lab and some of what we do in it.
We will soon be meeting every month, check back here for our 2019 restart. We have a list of frequently-asked questions.
We also maintain an outward facing website at BioHackspace.org
How to find us
- Email us here. You can also sign up to our mailing list where we do most of our communication here (if you see a warning ignore it) (Your posts will be moderated unless you're signed up to the list. If you want to be signed up to the list but not receive messages you can change this in the options). You can view the mailing list archives through the previous link or through the google groups interface. To chat in more detail request a signup to our slack channel using the email address above, or join our irc channel at #london-biohackers on freenode
Biohackspace Membership
We're a subgroup of the London Hackspace, and our rent and utility bills are paid as part of the larger space. Therefore it's London Biohackers also need to be members of the hackspace. You can find out how to become a hackspace member here.
There is a separate subscription for the biohacking group. Biohacking is more expensive than the typical hackspace activity, and with your membership we can pay for reagents, lab consumables and any equipment we may need for our experiments. The suggested minimum donation is £2 a week, but again, you can come along without joining. If you start to come regularly and use consumables then we'd like you to pay. Details on how to setup payment to become a biohackspace member are here: Details of Membership.
We also have an arrangement for people who just want to support the biohackspace work and lab costs, and do not intend to regularly attend the biolab to do science. If you want to become a remote member, or to donate equipment and make a regular contribution, and you can make contact here by email, or see the Donations/Remote members page for more details.
However it's not essential to become a member just to come along initially and find out what we do.
Organization
The biohackspace operates as a Company Limited by guarantee. The legal details of this are here
Projects
We are currently well equipped to carry out molecular biology experiments. The lab space on Hackney Road was a CL1 approved lab and we are aiming to reestablish that in Wembley.
Current Projects
- Hacking the lab
- * Recommissioning RO water system
- * Recommissioning Laminar Flow system
- * Building Laminar Flow System into a hood
- Culturing Local Algae
- Lab Automation
- Stirling Engine for Cryogenics
Completed Projects
- iGEM 2015
- Project:iGEM 2014
- Project:Flow Hood
- E. coli transformation
- Image Focus Stacking
- Lab Containment Level 1
- Growing V. fischeri
- Project:Sex typing with amelogenin
- Project:Public Biobrick (in collaboration with the UCL igem team)
- Blood typing
- Project:Plant species testing
- Project:Gel electrophoresis box
- Project:Shaker platform
Future Projects
- Project:Making our own reagents
- MindControlBacteria
- More and more becoming current project :)
Add your own!
Our lab
Our lab is small in size but we have tried to make good use of the tight space to incorporate all the equipment we need to carry out interesting projects. You can see a floor plan of the lab here.
We are currently working towards being are a certified Containment Level 1 Lab (more details on biosafety classifications).
The Lab is the registered office of our limited company, London Biological Laboratories, used to deal with suppliers.
How to use the Lab
Please do not touch things in the lab if you do not know what they are or how to use them. Many items may be hazards to your health if used incorrectly.
Our lab manual details our equipment, reagents, strains and our established protocols.
Starting your own lab?
We have some notes on starting your own biolab based on our experiences.
Equipment we made
Our hope is to be able to use self-developed or open source hardware equipment where possible in order to carry out our projects. Here is what we have done so far:
A list of biohacking hardware projects is here Project:Biohardware
Equipment Pledges
- Pledge: Flow Hood
- Pledge:Lab Microscope
- Pledge:vacum forming machine
- Pledge:Gluconacetobacter_hansenii
Resources
Education
- Nature.com comic about Synthetic Biology - Nice introduction to playing with genetic engineering/cloning, though aimed at kids
- Bugs' Crash Course in Molecular Biology - Recorded at the hackspace. Slides
- Sara's Introduction the the Biohacking Community - Recorded at the hackspace
A primer for synthetic biology
- Good introduction to gel electrophoresis - Explaining one of the core techniques for working with DNA
- Good introduction to the Polymerase Chain Reaction (PCR) - Explaining one of the core techniques for working with DNA
- Nature.com article on Biohacking - Free access. Nice explanation of what's needed in a lab and how different groups are managing it.
- Bioinformatics Resources
- Slides from Bioinformatics workshop August 2012
- Cathal Garvey's beginner's guide to biotech
- Flowchart diagram of DNA extraction protocol
Community
- Interesting DIYBio community blog - Interesting blog taking articles from a few different groups
- Bio Curious - A well-established biohacking group in California
- Brain-Computer interface at Paris hackspace
- Nature.com article about biohacking community - Not the same one as above. Interesting, but behind a paywall :(.
- you can access it here: http://www.synbioproject.org/process/assets/files/6452/_draft/nbt-2009-12d_-_biotech_nin_the_basement.pdf -- kanzure 70.114.205.110 19:40, 2 January 2011 (UTC)
Proposed code of conduct
See here: http://wiki.hackspace.org.uk/wiki/Biohacking/Code_of_Conduct