Difference between revisions of "HS Medium"
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== Info == | == Info == | ||
| − | Also known as Hestrin–Schramm medium. Used for growing [[Gluconacetobacter hansenii]]. | + | Also known as Hestrin–Schramm medium. Used for growing ''[[Gluconacetobacter hansenii]]''. |
== Protocol == | == Protocol == | ||
| − | # Using a balance weigh out the following and transfer to a 1 litre screwcap bottle: | + | # Using a balance weigh out the following and transfer to a '''1 litre''' screwcap bottle: |
| − | #* 20g D-glucose | + | #* '''20g D-glucose''' |
| − | #* 5g peptone | + | #* '''5g peptone''' |
| − | #* 5g yeast extract | + | #* '''5g yeast extract''' |
| − | #* 1.15g citrate | + | #* '''1.15g citrate''' |
| − | #* 2.7g disodium phosphate | + | #* '''2.7g disodium phosphate''' |
#* (15g agar if making solid medium for plates) | #* (15g agar if making solid medium for plates) | ||
# Half fill the bottle with water | # Half fill the bottle with water | ||
# Mix until all the additives are dissolved | # Mix until all the additives are dissolved | ||
| − | # Adjust pH | + | # Adjust to '''pH 6.0''' using the pH meter and adding drops of NaOH |
# Make up to 1 litre with water | # Make up to 1 litre with water | ||
# Autoclave | # Autoclave | ||
== Notes == | == Notes == | ||
| + | 1.15g citrate can be replaced with 25ml of lemon juice. | ||
| + | |||
| + | |||
| + | |||
| + | [[Category:Biohacking]] | ||
Latest revision as of 20:11, 14 October 2014
Info
Also known as Hestrin–Schramm medium. Used for growing Gluconacetobacter hansenii.
Protocol
- Using a balance weigh out the following and transfer to a 1 litre screwcap bottle:
- 20g D-glucose
- 5g peptone
- 5g yeast extract
- 1.15g citrate
- 2.7g disodium phosphate
- (15g agar if making solid medium for plates)
- Half fill the bottle with water
- Mix until all the additives are dissolved
- Adjust to pH 6.0 using the pH meter and adding drops of NaOH
- Make up to 1 litre with water
- Autoclave
Notes
1.15g citrate can be replaced with 25ml of lemon juice.