Pledge: Biohacking

Gene detection experiment

At a guesstimated total cost of £200.

This would give us the immediate means to do gene detection (by designing primers that mark particular genes.) E.g.:

• Detection of genetic modifications (identify GM food)
• Detection of species ("lamb curry test": are you getting what you bought?)
• Detection of variants of species (e.g. detect known traits based on published gene sequences)
• We can also send the results to a sequencing lab to get raw data back.

Pledges:

• martind £20
• ...

Details

The money would provide us with enough materials for dozens to hundreds of experiments (depending on the particular item.)

For PCR:

• Primers (£20)
• PCR Reagents (£40)
• Pipette & tips (£30)
• 0.5ml tubes (£10)

For electrophoresis:

• Power source (100V, low hundreds of mA; we have this)
• Agarose (£30)
• TBE Tablets (£10)
• Ladder (£20)
• Loading buffer (£20)
• Gel red/SYBR safe (£30)

PCR machine / Thermal cycler

ALL MONEY RECEIVED - THANKS! PLEASE DIRECT YOUR MONEY TO OTHER ITEMS ON OUR PLEDGE LIST.

We won the PCR machine for £120.

• Nigel £30 - PAID £15
• Gilda £40 - PAID £15
• Irwin £15 - PAID £15
• Lauren £15 - PAID £15
• Martind £100 - PAID £15, pls claim the rest from me once more purchases were made
• Jonty £15 - PAID £15
• Radek £30
• Bugs £30 - PAID £15
• Keith $100 USD

Total pledged: £275 + $100 from the colonies

Total needed: £120

PAID: £105

Incubator

This one has been bid on and should be OK, currently at £10 http://cgi.ebay.co.uk/220725833946

...might need a bit of hacking, but we're good at that