Difference between revisions of "Pledge: Biohacking"

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==Gene detection experiment==
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[[Category:Pledges]]
 
 
At a guesstimated total cost of £200.
 
 
 
Together with the equipment that we already have (thermal cycler) and are about to build (electrophoresis tank) this would give us the immediate means to do gene detection (we will design [http://en.wikipedia.org/wiki/Primer_(molecular_biology) primers] to extract particular genes.) E.g.:
 
* Detection of genetic modifications (identify [http://en.wikipedia.org/wiki/Genetically_modified_food GM food])
 
* Detection of species ("lamb curry test": are you getting what you bought?)
 
* Detection of variants of species (e.g. detect known traits based on published gene sequences)
 
* We can also send the results to a [http://en.wikipedia.org/wiki/Sequencing sequencing lab] to get raw data back.
 
 
 
'''Pledges:'''
 
* martind £50
 
* Andy Cousins (amc) £50
 
* Gilda £30
 
* Bugs £50
 
 
 
=== Details ===
 
 
 
The money would provide us with enough materials for dozens to hundreds of experiments (depending on the particular item.)
 
 
 
For [http://en.wikipedia.org/wiki/Polymerase_chain_reaction PCR]:
 
* Primers (£20)
 
* PCR Reagents (£40)
 
* Pipette & tips (£30)
 
* 0.5ml tubes (£10),
 
** [http://www.coleparmer.co.uk/catalog/product_view.asp?sku=0256031 Cole-Parmer, £44.00 for 500]
 
** [http://www.coleparmer.co.uk/catalog/product_view.asp?sku=0256032 Cole-Parmer, £21.10 for £50]
 
 
 
For [http://en.wikipedia.org/wiki/Electrophoresis electrophoresis]:
 
* Power source (100V, low hundreds of mA; we have this)
 
* Agarose (£30)
 
* TBE Tablets (£10)
 
* Ladder (£20)
 
* Loading buffer (£20)
 
* Gel red/SYBR safe (£30)
 
 
 
==PCR machine / Thermal cycler==
 
ALL MONEY RECEIVED - THANKS! PLEASE DIRECT YOUR MONEY TO OTHER ITEMS ON OUR PLEDGE LIST.
 
 
 
We [http://groups.google.com/group/london-hack-space/msg/253a37314f29da95? won] the PCR machine for £120.
 
 
 
* <s>Nigel £30</s> - '''PAID £15'''
 
* <s>Gilda £40</s> - '''PAID £15'''
 
* <s>Irwin £15</s> - '''PAID £15'''
 
* <s>Lauren £15</s> - '''PAID £15'''
 
* <s>Martind £100</s> - '''PAID £15''', pls claim the rest from me once more purchases were made
 
* <s>Jonty £15</s> - '''PAID £15'''
 
* Radek £30
 
* <s>Bugs £30</s> - '''PAID £15'''
 
* Keith $100 USD
 
 
 
'''Total pledged: £275''' + $100 from the colonies
 
 
 
'''Total needed: £120'''
 
 
 
'''PAID: £105'''
 
 
 
==Incubator==
 
 
 
This one has been bid on and should be OK, currently at £10
 
http://cgi.ebay.co.uk/220725833946
 
 
 
...might need a bit of hacking, but we're good at that
 

Latest revision as of 09:36, 16 May 2013