Difference between revisions of "Pledge: Biohacking"

From London Hackspace Wiki
Jump to: navigation, search
m
 
(4 intermediate revisions by 3 users not shown)
Line 1: Line 1:
==Gene detection experiment==
+
[[Category:Pledges]]
 
 
At a guesstimated total cost of £200.
 
 
 
Together with the equipment that we already have (thermal cycler) and are about to build (electrophoresis tank) this would give us the immediate means to do gene detection (we will design [http://en.wikipedia.org/wiki/Primer_(molecular_biology) primers] to extract particular genes.) E.g.:
 
* Detection of genetic modifications (identify [http://en.wikipedia.org/wiki/Genetically_modified_food GM food])
 
* Detection of species ("lamb curry test": are you getting what you bought?)
 
* Detection of variants of species (e.g. detect known traits based on published gene sequences)
 
* We can also send the results to a [http://en.wikipedia.org/wiki/Sequencing sequencing lab] to get raw data back.
 
 
 
==Pledges==
 
* martind £50
 
* Andy Cousins (amc) £50
 
* Gilda £30
 
* Bugs £50
 
* Mike £50
 
 
 
=== Details ===
 
====Estimates====
 
 
 
The money would provide us with enough materials for dozens to hundreds of experiments (depending on the particular item.)
 
 
 
For [http://en.wikipedia.org/wiki/Polymerase_chain_reaction PCR]:
 
* Primers (£20)
 
* PCR Reagents (£40)
 
** [http://www.sigmaaldrich.com/catalog/Lookup.do?N5=All&N3=mode+matchpartialmax&N4=pcr%20reagent&D7=0&D10=taq&N1=S_ID&ST=RS&N25=0&F=PR Simga-Aldrich has loads]
 
* 0.5ml tubes (£10),
 
** [http://www.coleparmer.co.uk/catalog/product_view.asp?sku=0256031 Cole-Parmer, £44.00 for 500]
 
** [http://www.coleparmer.co.uk/catalog/product_view.asp?sku=0256032 Cole-Parmer, £21.10 for £50]
 
 
 
====What we've bought so far:====
 
*[http://www.nbsbio.co.uk/product.asp?pID=8173&cID=70&c=1507 Agarose 100g] £38
 
*[http://www.nbsbio.co.uk/product.asp?pID=6241&cID=75 Safewhite 1ml] £30 (This is combined loading dye & DNA stain)
 
*[http://www.nbsbio.co.uk/product.asp?pID=10571&cID=33 DNA Ladder] £15
 
*[http://nbsbio.co.uk/product.asp?pID=1528&cID=4 TBE Concentrate] £10
 
 
 
* Shipping £10
 
* VAT £18.60
 
'''Total = 121.60'''
 
 
 
...This is everything necessary for [http://en.wikipedia.org/wiki/Electrophoresis electrophoresis of DNA] on an agarose gel, for checking the results of PCR reactions, etc.
 
 
 
*5-50 ul single channel pipette £12 (eBay seller [http://myworld.ebay.co.uk/fizzpinkfizz/ fizzpinkfizz])
 
*1000 2-200 ul Pipette tips £8.89 (eBay seller [http://myworld.ebay.co.uk/trafalgarscientific/?_trksid=p4340.l2559 trafalgarscientific])
 
 
 
==PCR machine / Thermal cycler==
 
ALL MONEY RECEIVED - THANKS! PLEASE DIRECT YOUR MONEY TO OTHER ITEMS ON OUR PLEDGE LIST.
 
 
 
We [http://groups.google.com/group/london-hack-space/msg/253a37314f29da95? won] the PCR machine for £120.
 
==Pledges==
 
* <s>Nigel £30</s> - '''PAID £15'''
 
* <s>Gilda £40</s> - '''PAID £15'''
 
* <s>Irwin £15</s> - '''PAID £15'''
 
* <s>Lauren £15</s> - '''PAID £15'''
 
* <s>Martind £100</s> - '''PAID £15''', pls claim the rest from me once more purchases were made
 
* <s>Jonty £15</s> - '''PAID £15'''
 
* Radek £30
 
* <s>Bugs £30</s> - '''PAID £15'''
 
* Keith $100 USD
 
 
 
Running total: '''£275'''
 
 
 
Total needed: '''£120'''
 
 
 
Paid: '''£105'''
 
 
 
==Incubator==
 
 
 
This one has been bid on and should be OK, currently at £10
 
http://cgi.ebay.co.uk/220725833946
 
 
 
...might need a bit of hacking, but we're good at that
 
 
 
[[category:pledge]]
 

Latest revision as of 09:36, 16 May 2013