Pledge: Biohacking

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Revision as of 23:16, 2 March 2011 by Martind (talk | contribs) (Details)
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Gene detection experiment

At a guesstimated total cost of £200.

This would give us the immediate means to do gene detection (by designing primers that mark particular genes.) E.g.:

  • Detection of genetic modifications (identify GM food)
  • Detection of species ("lamb curry test": are you getting what you bought?)
  • Detection of variants of species (e.g. detect known traits based on published gene sequences)
  • We can also send the results to a sequencing lab to get raw data back.

Pledges:

  • martind £20
  • ...

Details

The money would provide us with enough materials for dozens to hundreds of experiments (depending on the particular item.)

For PCR:

  • Primers (£20)
  • PCR Reagents (£40)
  • Pipette & tips (£30)
  • 0.5ml tubes (£10)

For electrophoresis:

  • Power source (100V, low hundreds of mA; we have this)
  • Agarose (£30)
  • TBE Tablets (£10)
  • Ladder (£20)
  • Loading buffer (£20)
  • Gel red/SYBR safe (£30)

PCR machine / Thermal cycler

ALL MONEY RECEIVED - THANKS! PLEASE DIRECT YOUR MONEY TO OTHER ITEMS ON OUR PLEDGE LIST.

We won the PCR machine for £120.

  • Nigel £30 - PAID £15
  • Gilda £40 - PAID £15
  • Irwin £15 - PAID £15
  • Lauren £15 - PAID £15
  • Martind £100 - PAID £15, pls claim the rest from me once more purchases were made
  • Jonty £15 - PAID £15
  • Radek £30
  • Bugs £30 - PAID £15
  • Keith $100 USD

Total pledged: £275 + $100 from the colonies

Total needed: £120

PAID: £105

Incubator

This one has been bid on and should be OK, currently at £10 http://cgi.ebay.co.uk/220725833946

...might need a bit of hacking, but we're good at that