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Make and run an agarose gel: Difference between revisions
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*Once gel is set, remove tape and place in outer box. | *Once gel is set, remove tape and place in outer box. | ||
* Make loading buffer (about 100ml for a 50ml gel), of 1xTBE. This lets the current flow. | * Make loading buffer (about 100ml for a 50ml gel), of 1xTBE. This lets the current flow. | ||
* Pour loading buffer over gel so gel is completely covered by | * Pour loading buffer over gel so gel is completely covered by 2-5 mm of buffer. | ||
=== Load samples, run gel and visualise: === | === Load samples, run gel and visualise: === | ||
