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Electrophoresis Stains: Difference between revisions
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* Cost of disposal: £125 per 60 litres. Therefore minimum cost with 50ml gels is 125 / (60000 / 50) = £0.104 a gel | * Cost of disposal: £125 per 60 litres. Therefore minimum cost with 50ml gels is 125 / (60000 / 50) = £0.104 a gel | ||
* Cost, total: 10p per gel, perhaps more realistically 15p-20p per per gel (since we'll want to put other stuff in the bins). | * Cost, total: 10p per gel, perhaps more realistically 15p-20p per per gel (since we'll want to put other stuff in the bins). | ||
* Usage: | * Usage: flouresces under UV at 254 nm wavelength. | ||
* Other considerations: possible mutagen, carcinogen, and teratogen. | * Other considerations: possible mutagen, carcinogen, and teratogen. (This is from tests on cultured cell lines. There is no direct evidence implicating EtBr as a carcinogen in any animal. | ||
* Why it's viable: We know it works. It's quite cheap. We have to organise professional hazardous waste disposal anyway. | * Why it's viable: We know it works. It's quite cheap. We have to organise professional hazardous waste disposal anyway. | ||
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* Cost of product: £40 for 1ml. 2ul per reaction, therefore 40 / (1000 / 2) = £0.08 a reaction | * Cost of product: £40 for 1ml. 2ul per reaction, therefore 40 / (1000 / 2) = £0.08 a reaction | ||
* Cost, total: 8p per reaction, assuming 4 reactions per gel = 32p per gel. | * Cost, total: 8p per reaction, assuming 4 reactions per gel = 32p per gel. | ||
* Usage: Add to samples not gel. Fluorescence excitation maxima at approx 290-320nm, emitting at 515nm | |||
* Other considerations: must be refrigerated (4C). | * Other considerations: must be refrigerated (4C). | ||
* Why it's viable: Safe. Relatively cheap. Can be purchased in bulk for a saving of 1p per sample. | * Why it's viable: Safe. Relatively cheap. Can be purchased in bulk for a saving of 1p per sample. | ||
=== SafeView === | |||
* Info: http://www.nbsbio.co.uk/product.asp?pID=2490&cID=71 | |||
* Cost of product: £40 for 1ml. | |||
* Usage: add to gel as with EtBr. Fluorescence excitation maxima at approx 290-320nm, emitting at 515nm | |||
=== GelGreen or GelRed === | === GelGreen or GelRed === | ||
