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Project:Blood typing: Difference between revisions
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→Extraction and PCR (Will, Simon, Lui) 12/2/13
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* 25ul total PCR reaction volume - 5ul template (1ul for PC), 2.5ul each forward & reverse primers, 12.5ul Taq readymix, 2.5ul dH20 (6.5ul for PC) | * 25ul total PCR reaction volume - 5ul template (1ul for PC), 2.5ul each forward & reverse primers, 12.5ul Taq readymix, 2.5ul dH20 (6.5ul for PC) | ||
* Initial D - 96C 5 mins, then 35 cycles of D-96C 1 min, A-55C 30 secs, E-72C 1 min. | * Initial D - 96C 5 mins, then 35 cycles of D-96C 1 min, A-55C 30 secs, E-72C 1 min. | ||
[[File:gel12feb2013.jpg|left|400px|thumb|none|Gel 12 Feb]] | [[File:gel12feb2013.jpg|left|400px|thumb|none|Gel 12 Feb, taken with Samsung Galaxy S3 in night mode]] | ||
* 15ul of samples loaded, 3ul loading buffer, 7ul of ladder loaded. | * 15ul of samples loaded, 3ul loading buffer, 7ul of ladder loaded. | ||
'''From left (after 40 mins at 80V:)''' | '''From left (after 40 mins at 80V:)''' | ||
