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Project:Blood typing: Difference between revisions
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→Extraction and PCR (David, Will) 17/2/13
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* Initial D - 96C 5 mins, then 40 cycles of D-96C 1 min, A-55C 30 secs, E-72C 1 min. | * Initial D - 96C 5 mins, then 40 cycles of D-96C 1 min, A-55C 30 secs, E-72C 1 min. | ||
[[File:gel17feb2013.jpg|left|400px|thumb|none|Gel 17 Feb]] | [[File:gel17feb2013.jpg|left|400px|thumb|none|Gel 17 Feb]] | ||
* 15ul of samples loaded | * 25ul of PCR samples loaded, 15ul of gDNA samples loaded (2ul for PC gDNA). 2ul loading buffer, 5ul of ladder loaded. | ||
'''From left (after 40 mins at 80V:)''' | '''From left (after 40 mins at 80V:)''' | ||
*1: 100-3000bp ladder. Ladder [http://www.nbsbio.co.uk/product.asp?pID=8951&cID=33 key]. | *1: 100-3000bp ladder. Ladder [http://www.nbsbio.co.uk/product.asp?pID=8951&cID=33 key]. | ||
