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Project:Blood typing: Difference between revisions
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→Extraction and PCR (David, Will) 17/2/13
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* 25ul total PCR reaction volume - 2.5ul dH20 (6.5ul for PC), 5ul template (1ul for PC), 2.5ul each forward & reverse primers, 12.5ul Taq readymix | * 25ul total PCR reaction volume - 2.5ul dH20 (6.5ul for PC), 5ul template (1ul for PC), 2.5ul each forward & reverse primers, 12.5ul Taq readymix | ||
* Initial D - 96C 5 mins, then 40 cycles of D-96C 1 min, A-55C 30 secs, E-72C 1 min. | * Initial D - 96C 5 mins, then 40 cycles of D-96C 1 min, A-55C 30 secs, E-72C 1 min. | ||
[[File:gel17feb2013. | [[File:gel17feb2013.JPG|left|400px|thumb|none|Gel 17 Feb]] | ||
* 25ul of PCR samples loaded, 15ul of gDNA samples loaded (2ul for PC gDNA). 2ul loading buffer, 5ul of ladder loaded. | * 25ul of PCR samples loaded, 15ul of gDNA samples loaded (2ul for PC gDNA). 2ul loading buffer, 5ul of ladder loaded. | ||
* We expect to see PCR bands at 784bp. | * We expect to see PCR bands at 784bp. | ||
