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Project:Blood typing: Difference between revisions
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→Extraction and PCR (JJ, Bene, Will) 20-23/13
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* 50ul total PCR reaction volume - 5ul dH20 (13ul for PC), 10ul template (2ul for PC), 5ul each forward & reverse primers, 25ul Taq readymix | * 50ul total PCR reaction volume - 5ul dH20 (13ul for PC), 10ul template (2ul for PC), 5ul each forward & reverse primers, 25ul Taq readymix | ||
* Initial D - 96C 5 mins, then 40 cycles of D-96C 1 min, A-55C 30 secs, E-72C 1 min. | * Initial D - 96C 5 mins, then 40 cycles of D-96C 1 min, A-55C 30 secs, E-72C 1 min. | ||
[[File:gel1-23feb2013.JPG|left| | [[File:gel1-23feb2013.JPG|left|300px|thumb|none|Gel 23 Feb]] | ||
* 20ul of PCR samples loaded, 20ul of gDNA samples loaded. 4ul loading buffer. | * 20ul of PCR samples loaded, 20ul of gDNA samples loaded. 4ul loading buffer. | ||
*1: JJ PCR product (no band) | *1: JJ PCR product (no band) | ||
