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Electrophoresis Stains: Difference between revisions
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→SYBR Safe
Mycoplasma (talk | contribs) |
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* Total cost: 55p per gel. | * Total cost: 55p per gel. | ||
* Why it's not viable: Invitrogen won't sell anything to us. (but we can retry with them) | * Why it's not viable: Invitrogen won't sell anything to us. (but we can retry with them) | ||
* Our experience: We tested 5ul of sybrsafe that was donated to us, and found it to give much fainter bands than EtBr. See below | * Our experience: We tested 5ul of sybrsafe that was donated to us, and found it to give much fainter bands than EtBr. See below. Lane 6 on the EtBr gel and lane 4 on the sybr safe gel is 2ul of the cell culture gDNA we use as a positive control. The difference is clear. The remaining numbered lanes on each gel are either ladder, PCR products from our extractions or our gDNA extractions. None of these were visible on the sybr safe gel. | ||
[[File:gel17feb2013.JPG|left|400px|thumb|none|EtBr gel]][[File:Sybrsafegel1mar2013.jpg|400px|thumb|none|SYBR Safe gel]] | [[File:gel17feb2013.JPG|left|400px|thumb|none|EtBr gel]][[File:Sybrsafegel1mar2013.jpg|400px|thumb|none|SYBR Safe gel]] | ||
<br style="clear: both" /> | <br style="clear: both" /> | ||
