Transforming bacteria: Difference between revisions

Transforming bacteria (view source)

10 bytes added , 11 September 2013

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Revision as of 20:26, 11 September 2013 (view source) Mycoplasma (talk \| contribs) (→‎Replating Wed 4 sept 2013) ← Older edit		Revision as of 21:59, 11 September 2013 (view source) Mycoplasma (talk \| contribs) (→‎Transformation Wed 11 sept 2013) Newer edit →
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	===Transformation Wed 11 sept 2013===		===Transformation Wed 11 sept 2013===
	* Incubated 50ul of competent ~~cells~~ from 9-11 with 2ul PUC19 for 30 mins on ice (also control with no PUC19 added). Then 42C for 45 seconds. 2 mins back on ice. Added 500ul LB. Incubated at 37C for 1 hr. Plated on amp plates and incubated at 37C overnight.		* Incubated 50ul of competent DH5α from 2013-4-11 with 2ul PUC19 for 30 mins on ice (also control with no PUC19 added). Then 42C for 45 seconds. 2 mins back on ice. Added 500ul LB. Incubated at 37C for 1 hr. Plated on amp plates and incubated at 37C overnight.