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LBL07001: Difference between revisions
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(Created page with " == Preparation of competent E. Coli (chemical method) == {| class="wikitable" |Author: S. Thompson |Approved by: |SOP No. LBL07001 |- |Signed: |Signed: |Effective from: |- |D...") |
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'''4. Materials''' | '''4. Materials''' | ||
E. Coli. (plate colony or glycerol stock) | E. Coli. (plate colony or glycerol stock)<br> | ||
Ice bucket. | Ice bucket.<br> | ||
0.1M CaCl. | 0.1M CaCl.<br> | ||
0.1M MgCl | 0.1M MgCl.<br> | ||
50mL centrifuge tubes. | 50mL centrifuge tubes.<br> | ||
15mL centrifuge tubes. | 15mL centrifuge tubes.<br> | ||
1.5-2.0mL eppendorf tubes. | 1.5-2.0mL eppendorf tubes.<br> | ||
LB broth (100mL in sterile erhlenmeyer flask) | LB broth (100mL in sterile erhlenmeyer flask)<br> | ||
Incubator (shaking) 37C | Incubator (shaking) 37C <br> | ||
'''5. Related documents''' | '''5. Related documents''' | ||
LB broth preparation | LB broth preparation<br> | ||
LB Agar selection media preparation | LB Agar selection media preparation<br> | ||
E. coli heat shock tranformation | E. coli heat shock tranformation<br> | ||
Rapid transformation | Rapid transformation<br> | ||
'''6. Definitions''' | '''6. Definitions''' | ||
