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* Reaction volume - 12.5ul Taq, 5ul template, 5ul primers, 2.5ul deionised water. ...s assumed as no record: 5ul template (1ul for PC), 5ul primers, 12.5ul Taq readymix, 2.5ul dH20 (6.5ul for PC).

...for the readymix one, but not for the mastermix one, so proceeded with the readymix one. ...ent/Thermal_Cycler_(Perkin_Elmer_480) thermocycler] and our standard [[taq readymix]]. The major difference is that we usually have a D step of 30 sec, whereas

Assume 25ul total volume. 12.5ul Taq Readymix, 5ul primers * Taq Readymix: £0.72 (12.5ul)

=== SYBR Green as part of a readymix === * Cost of product: £96.60 for 100 reactions, so £0.96 per gel, BUT Taq readymix itself costs around £100 for 100 reactions, so it is essentially free.

* 25 ul of fast-start PCR Taq readymix ...10 mM Tris.HCl pH 8.3, 4.0 mM MgCl_2, 120 pmoles of each primer, and 2.5 U Taq polymerase. PCR was run for 30 cycles of 94 C for 1 min (denature), 65 C fo