Make and run an agarose gel: Difference between revisions
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* Choose the gel concentration (higher the concentration the smaller the bands you can see). The following instructions are for a 1% of 50ml, which we normally use. | * Choose the gel concentration (higher the concentration the smaller the bands you can see). The following instructions are for a 1% of 50ml, which we normally use. | ||
* Add 1 gram of agarose to 5g (or ml) of TBE 10x and 45g (or ml) of dH20. | * Add 1 gram of agarose to 5g (or ml) of TBE 10x and 45g (or ml) of dH20 (we use deionized water). | ||
* Mix and then microwave this until it is clear (normally around 1 min) | * Mix and then microwave this until it is clear (normally around 1 min) | ||
* Leave to cool, then add Ethidium Bromide stock solution at a concentration of 2ul/40ml (so about 2.25ul for this gel) at about 50°C. | * Leave to cool, then add Ethidium Bromide stock solution at a concentration of 2ul/40ml (so about 2.25ul for this gel) at about 50°C. | ||
Revision as of 20:05, 4 September 2012
Make gel
- Choose the gel concentration (higher the concentration the smaller the bands you can see). The following instructions are for a 1% of 50ml, which we normally use.
- Add 1 gram of agarose to 5g (or ml) of TBE 10x and 45g (or ml) of dH20 (we use deionized water).
- Mix and then microwave this until it is clear (normally around 1 min)
- Leave to cool, then add Ethidium Bromide stock solution at a concentration of 2ul/40ml (so about 2.25ul for this gel) at about 50°C.
