Project:Growing bacteria: Difference between revisions
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Two plates of v. fischeri brought to lab. They are bioluminescent and glow green in the dark. When we got them they had been growing for a few days on 'BOSS' media (BOSS medium: 1% peptone, 0.3% beef extract, 0.1% glycerol, 3% NaCl, 1.5% agar, pH 7.3) | Two plates of v. fischeri brought to lab. They are bioluminescent and glow green in the dark. When we got them they had been growing for a few days on 'BOSS' media (BOSS medium: 1% peptone, 0.3% beef extract, 0.1% glycerol, 3% NaCl, 1.5% agar, pH 7.3) | ||
[[File:Glowing_plates.jpg|200px|thumb|left|Photo by Raphael Kim]] | [[File:Glowing_plates.jpg|200px|thumb|left|Photo by Raphael Kim]] | ||
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=== 9th march === | === 9th march === | ||
Some cultures transferred to new plates. [http://wiki.london.hackspace.org.uk/view/File:Vibrio_Fischeri_Culture_Protocol_9.3.pdf Protocol] | Some cultures transferred to new plates. [http://wiki.london.hackspace.org.uk/view/File:Vibrio_Fischeri_Culture_Protocol_9.3.pdf Protocol] | ||
Revision as of 16:46, 15 March 2013
V. Fischeri
7th march
Two plates of v. fischeri brought to lab. They are bioluminescent and glow green in the dark. When we got them they had been growing for a few days on 'BOSS' media (BOSS medium: 1% peptone, 0.3% beef extract, 0.1% glycerol, 3% NaCl, 1.5% agar, pH 7.3)
9th march
Some cultures transferred to new plates. Protocol
10th march
More cultures transferred to new plates. Protocol
13th march
Original cultures still glow, but nothing from the new ones
E. Coli
Equipment
We need to fix our incubator with shaker, or make a new one.
Requirements
- Easily cleaned + sterilised
- Either a shaker powerful enough to agitate full beakers, or a magnetic stirrer (which we have already started building). Magnetic stirrer may lyse cells. An idea for a shaker could be a record player.
- Can be made dark inside
- Adjustable temp
- Low power if possible.
- Temperature mesaurement (simple thermometer would do)
- Does it need to be sealable?
