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Project:Growing bacteria: Difference between revisions
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===16th march=== | ===16th march=== | ||
Culture A still ok, culture B almost disappeared. | Culture A still ok, culture B almost disappeared. | ||
Two cultures of ''V. fischeri'', two cultures of ''E. coli'', and one culture of 'biohackers thumb prints' were plated onto an approximation of BOSS media. Media was 4g table salt, 5g LB (containing peptone, salt and yeast), 0.2g glycerol, 3g agar in 200ml water. (3% nacl, 1% peptone, 0.1% glycerol, 0.5% yeast extract, 1.5% agar). Some cultures of ''V. fischeri'' from original plate A were frozen in the same medium minus the agar, and with 3% glycerol instead of 0.1%. | Two cultures of ''V. fischeri'', two cultures of ''E. coli'', and one culture of 'biohackers thumb prints' were plated onto an approximation of BOSS media. Media was 4g table salt, 5g LB (containing peptone, salt and yeast), 0.2g glycerol, 3g agar in 200ml water. (3% nacl, 1% peptone, 0.1% glycerol, 0.5% yeast extract, 1.5% agar). Some cultures of ''V. fischeri'' from original plate A were frozen in the same medium minus the agar, and with 3% glycerol instead of 0.1%. Cultures stored at room temp, although ''E. coli'' incubated at 37C for a couple of hours first. | ||
== E. Coli == | == E. Coli == | ||
