LB Medium: Difference between revisions
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Revision as of 01:47, 13 September 2014
Info
Used for growing E. coli.
Protocol
- Using a balance weigh out the following and transfer to a 1 litre screwcap bottle:
- 25g LB powder
- (15g agar if making solid medium for plates)
- Half fill the bottle with water
- Mix until all the additives are dissolved
- Adjust to pH 7.0 using the pH meter and adding drops of NaOH or HCl.
- Make up to 1 litre with water
- Autoclave
Notes
If adding antibiotic, add after autoclaving.
25g of LB powder consists of:
- 10g tryptone
- 5g yeast extract
- 10g NaCl
