LB Medium: Difference between revisions
From London Hackspace Wiki
(Created page with "== Info == Used for growing ''E. coli''. == Protocol == # Using a balance weigh out the following and transfer to a '''1 litre''' screwcap bottle: #* '''2...") |
Mycoplasma (talk | contribs) No edit summary |
||
| (3 intermediate revisions by 2 users not shown) | |||
| Line 6: | Line 6: | ||
# Using a balance weigh out the following and transfer to a '''1 litre''' screwcap bottle: | # Using a balance weigh out the following and transfer to a '''1 litre''' screwcap bottle: | ||
#* '''25g LB powder''' | #* '''25g LB-Miller powder''' *or* '''20g LB-Lennox powder''' *or* '''15.5g LB-Luria powder''' | ||
#* (15g agar if making solid medium for plates) | #* (15g agar if making solid medium for plates) | ||
# Half fill the bottle with water | # Half fill the bottle with water | ||
| Line 17: | Line 17: | ||
If adding antibiotic, add after autoclaving. | If adding antibiotic, add after autoclaving. | ||
25g of LB powder consists of: | 25g of LB-Miller powder consists of: | ||
* 10g tryptone | |||
* 5g yeast extract | |||
* 10g NaCl | |||
20g of LB-Lennox powder consists of: | |||
* 10g tryptone | |||
* 5g yeast extract | |||
* 5g NaCl | |||
15.5g of LB-Luria powder consists of: | |||
* 10g tryptone | |||
* 5g yeast extract | |||
* 0.5g NaCl | |||
== With ampicillin == | |||
* 100ug/ml | |||
* Our aliquots are currently for use at 100ul/100ml | |||
[[Category:Biohacking]] | |||
Latest revision as of 17:13, 28 March 2015
Info
Used for growing E. coli.
Protocol
- Using a balance weigh out the following and transfer to a 1 litre screwcap bottle:
- 25g LB-Miller powder *or* 20g LB-Lennox powder *or* 15.5g LB-Luria powder
- (15g agar if making solid medium for plates)
- Half fill the bottle with water
- Mix until all the additives are dissolved
- Adjust to pH 7.0 using the pH meter and adding drops of NaOH or HCl.
- Make up to 1 litre with water
- Autoclave
Notes
If adding antibiotic, add after autoclaving.
25g of LB-Miller powder consists of:
- 10g tryptone
- 5g yeast extract
- 10g NaCl
20g of LB-Lennox powder consists of:
- 10g tryptone
- 5g yeast extract
- 5g NaCl
15.5g of LB-Luria powder consists of:
- 10g tryptone
- 5g yeast extract
- 0.5g NaCl
With ampicillin
- 100ug/ml
- Our aliquots are currently for use at 100ul/100ml
