Pledge: Biohacking: Difference between revisions
From London Hackspace Wiki
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At a guesstimated total cost of £200. | At a guesstimated total cost of £200. | ||
This would give us the immediate means to do gene detection ( | This would give us the immediate means to do gene detection (we will design [http://en.wikipedia.org/wiki/Primer_(molecular_biology) primers] to extract particular genes.) E.g.: | ||
* Detection of genetic modifications (identify [http://en.wikipedia.org/wiki/Genetically_modified_food GM food]) | * Detection of genetic modifications (identify [http://en.wikipedia.org/wiki/Genetically_modified_food GM food]) | ||
* Detection of species ("lamb curry test": are you getting what you bought?) | * Detection of species ("lamb curry test": are you getting what you bought?) | ||
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For [http://en.wikipedia.org/wiki/Polymerase_chain_reaction PCR]: | For [http://en.wikipedia.org/wiki/Polymerase_chain_reaction PCR]: | ||
* | * Primers (£20) | ||
* PCR Reagents (£40) | * PCR Reagents (£40) | ||
* Pipette & tips (£30) | * Pipette & tips (£30) | ||
Revision as of 23:17, 2 March 2011
Gene detection experiment
At a guesstimated total cost of £200.
This would give us the immediate means to do gene detection (we will design primers to extract particular genes.) E.g.:
- Detection of genetic modifications (identify GM food)
- Detection of species ("lamb curry test": are you getting what you bought?)
- Detection of variants of species (e.g. detect known traits based on published gene sequences)
- We can also send the results to a sequencing lab to get raw data back.
Pledges:
- martind £20
- ...
Details
The money would provide us with enough materials for dozens to hundreds of experiments (depending on the particular item.)
For PCR:
- Primers (£20)
- PCR Reagents (£40)
- Pipette & tips (£30)
- 0.5ml tubes (£10)
For electrophoresis:
- Power source (100V, low hundreds of mA; we have this)
- Agarose (£30)
- TBE Tablets (£10)
- Ladder (£20)
- Loading buffer (£20)
- Gel red/SYBR safe (£30)
PCR machine / Thermal cycler
ALL MONEY RECEIVED - THANKS! PLEASE DIRECT YOUR MONEY TO OTHER ITEMS ON OUR PLEDGE LIST.
We won the PCR machine for £120.
Nigel £30- PAID £15Gilda £40- PAID £15Irwin £15- PAID £15Lauren £15- PAID £15Martind £100- PAID £15, pls claim the rest from me once more purchases were madeJonty £15- PAID £15- Radek £30
Bugs £30- PAID £15- Keith $100 USD
Total pledged: £275 + $100 from the colonies
Total needed: £120
PAID: £105
Incubator
This one has been bid on and should be OK, currently at £10 http://cgi.ebay.co.uk/220725833946
...might need a bit of hacking, but we're good at that
