Difference between revisions of "Project:Growing bacteria"

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We need to fix our incubator with shaker, or make a new one.
 
We need to fix our incubator with shaker, or make a new one.
 
====Requirements====
 
====Requirements====
 +
* Easily cleaned + sterilised
 
* Shaker is powerful enough to agitate full beakers
 
* Shaker is powerful enough to agitate full beakers
 
* Can be made dark inside
 
* Can be made dark inside

Revision as of 00:21, 15 March 2013

V. Fischeri

7th march

Two plates of v. fischeri brought to lab. They are bioluminescent and glow green in the dark. When we got them they had been growing for a few days on 'BOSS' media (BOSS medium: 1% peptone, 0.3% beef extract, 0.1% glycerol, 3% NaCl, 1.5% agar, pH 7.3)

9th march

Some cultures transferred to new plates. Protocol

13th march

Original cultures still glow, but nothing from the new ones

E. Coli

Equipment

We need to fix our incubator with shaker, or make a new one.

Requirements

  • Easily cleaned + sterilised
  • Shaker is powerful enough to agitate full beakers
  • Can be made dark inside
  • Adjustable temp
  • Low power if possible.
  • Temperature mesaurement (simple thermometer would do)
  • Does it need to be sealable?