Pledge: Biohacking: Difference between revisions

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== Pledges for the Biohacking group ==
== Pledges for the Biohacking group ==


===Gene detection experiment===


====PCR machine / Thermal cycler====
With guesstimated cost.
 
For [http://en.wikipedia.org/wiki/Polymerase_chain_reaction PCR]:
* Primers (£20)
* PCR Reagents (£40)
* Pipette & tips (£30)
* 0.5ml tubes (£10)
 
For [http://en.wikipedia.org/wiki/Electrophoresis electrophoresis]:
* Power source (100V, low hundreds of mA; we have this)
* Agarose (£30)
* TBE Tablets (£10)
* Ladder (£20)
* Loading buffer (£20)
* Gel red/SYBR safe (£30)
 
This would give us the immediate means to do gene detection (by designing primers that mark particular genes.) E.g.:
* Detection of species ("lamb curry test")
* Detection of variants of species (e.g. detect known traits based on published gene sequences)
* Detection of genetic modifications (GMO food)
* We can also send the results to a sequencing lab to get data back.
 
'''Pledges:'''
* martind £20
* ...
 
===PCR machine / Thermal cycler===
ALL MONEY RECEIVED - THANKS! PLEASE DIRECT YOUR MONEY TO OTHER ITEMS ON OUR PLEDGE LIST.
ALL MONEY RECEIVED - THANKS! PLEASE DIRECT YOUR MONEY TO OTHER ITEMS ON OUR PLEDGE LIST.



Revision as of 22:02, 2 March 2011

Pledges for the Biohacking group

Gene detection experiment

With guesstimated cost.

For PCR:

  • Primers (£20)
  • PCR Reagents (£40)
  • Pipette & tips (£30)
  • 0.5ml tubes (£10)

For electrophoresis:

  • Power source (100V, low hundreds of mA; we have this)
  • Agarose (£30)
  • TBE Tablets (£10)
  • Ladder (£20)
  • Loading buffer (£20)
  • Gel red/SYBR safe (£30)

This would give us the immediate means to do gene detection (by designing primers that mark particular genes.) E.g.:

  • Detection of species ("lamb curry test")
  • Detection of variants of species (e.g. detect known traits based on published gene sequences)
  • Detection of genetic modifications (GMO food)
  • We can also send the results to a sequencing lab to get data back.

Pledges:

  • martind £20
  • ...

PCR machine / Thermal cycler

ALL MONEY RECEIVED - THANKS! PLEASE DIRECT YOUR MONEY TO OTHER ITEMS ON OUR PLEDGE LIST.

We won the PCR machine for £120.

  • Nigel £30 - PAID £15
  • Gilda £40 - PAID £15
  • Irwin £15 - PAID £15
  • Lauren £15 - PAID £15
  • Martind £100 - PAID £15, pls claim the rest from me once more purchases were made
  • Jonty £15 - PAID £15
  • Radek £30
  • Bugs £30 - PAID £15
  • Keith $100 USD

Total pledged: £275 + $100 from the colonies

Total needed: £120

PAID: £105

Incubator

This one has been bid on and should be OK, currently at £10 http://cgi.ebay.co.uk/220725833946

...might need a bit of hacking, but we're good at that