Project:Sex typing with amelogenin/results-20120212: Difference between revisions
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* DNA extraction: perhaps try several things, but especially: | * DNA extraction: perhaps try several things, but especially: | ||
** Detergent | |||
** Salt | |||
* DNA extraction: try centrifuging DNA | * DNA extraction: try centrifuging DNA | ||
* DNA extraction: perhaps test by combining some extracted DNA with dilute EtBr solution. | * DNA extraction: perhaps test by combining some extracted DNA with dilute EtBr solution. | ||
* Primers: can we tell that they are there? Try combining some with EtBr and viewing. | * Primers: can we tell that they are there? Try combining some with EtBr and viewing. | ||
* PCR: Do tests: | * PCR: Do tests: | ||
** Without primers or template | |||
** Without template | |||
** Without master mix (?) | |||
* PCR: Follow Wikipedia's basic melt, anneal, extend temperatures and times. | * PCR: Follow Wikipedia's basic melt, anneal, extend temperatures and times. | ||
Revision as of 20:54, 11 February 2012
To think about
- DNA extraction: perhaps try several things, but especially:
- Detergent
- Salt
- DNA extraction: try centrifuging DNA
- DNA extraction: perhaps test by combining some extracted DNA with dilute EtBr solution.
- Primers: can we tell that they are there? Try combining some with EtBr and viewing.
- PCR: Do tests:
- Without primers or template
- Without template
- Without master mix (?)
- PCR: Follow Wikipedia's basic melt, anneal, extend temperatures and times.
