Difference between revisions of "DNA extraction using Chelex 100"
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Mycoplasma (talk | contribs) (Created page with "* Fill half a tube (500ul) with chelex 100 (try and get 50% beads and 50% clear liquid) * Scrape off some cheek cells into the end of a pipette tip, add this and some saliva to t...") |
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| − | * Fill half a tube (500ul) with chelex 100 (try and get 50% beads and 50% clear liquid) | + | * Fill half a tube (500ul) with 5% chelex 100 (try and get 50% beads and 50% clear liquid) |
* Scrape off some cheek cells into the end of a pipette tip, add this and some saliva to the tube and mix | * Scrape off some cheek cells into the end of a pipette tip, add this and some saliva to the tube and mix | ||
* Cover with a drop of paraffin Incubate at 56°C for 30 mins, then at 94°C for 10 mins | * Cover with a drop of paraffin Incubate at 56°C for 30 mins, then at 94°C for 10 mins | ||
* Centrifuge briefly and take supernatant | * Centrifuge briefly and take supernatant | ||
* Store at -20°C | * Store at -20°C | ||
Revision as of 00:28, 24 August 2012
- Fill half a tube (500ul) with 5% chelex 100 (try and get 50% beads and 50% clear liquid)
- Scrape off some cheek cells into the end of a pipette tip, add this and some saliva to the tube and mix
- Cover with a drop of paraffin Incubate at 56°C for 30 mins, then at 94°C for 10 mins
- Centrifuge briefly and take supernatant
- Store at -20°C