LBL08001: Difference between revisions

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= LBL08001 - Maintenance and Cleaning =
== Maintenance and Cleaning ==
 
{| class="wikitable"
 
|Author:
 
|Approved by: S. Thompson
 
|SOP No. LBL08001


{|
|width="31%"|Author: Tom Hodder
|width="39%"|Approved by: S. Thompson
|width="28%"|SOP No. LBL08001
|-
|Signed:
|Signed:
|Effective from:
|-
|-
|Date:
|Date:
|Last edited:
|}


|Signed:
== Purpose ==


|Signed:
Maintenance and Cleaning are fundemental to the operation of a safe and successful biolab. This document provides the daily, weekly and ad-hoc procedures that are in place.


|Effective from:
== Scope ==


|-
* This SOP applies to all users in all circumstances in the LBL biolab without exception.
* In the case of specific instructions for Maintenance and Cleaning of equipment the user should defer to the relevant SOP for that particular piece of equipment.


|Date:
== Responsibilities ==


|Date:
* All users of the biolab are responsible for ensuring the clean and safe operation of equipment, lab furniture and tools.
* The Biological Safety Officer has oversight and responsilibity to make final decisions regarding maintenance and cleaning.


|Last edited:
There are risks associated with certain cleaning products. Bleach is considered a hazardous substance and care must be taken to follow the instructions on the label.


|}
It is the user's responsibility to check the MSDS for any chemical or cleaning products they are using.


'''1. Purpose'''
== Materials ==


**  general description of the SOP purpose
The following items should be available in the lab


'''2. Scope'''
* Disposable Nitrile gloves in various sizes
* Plastic aprons
* Eye protection
* Plastic over-shoe protection
* Cleaning products are available under the sink
* A Mop and bucket
* Blue roll
* Disinfectant
* Sterilzing Spray


**  the extent of the SOP in terms of to what equipment and procedures it applies, also when and where (which will always be at all times in the LBL lab).
== Related documents ==


'''3. Responsibilities'''
[[lbl08003.rst|lbl08003 - Handling spillage of micro-organisms]]<br />[[lbl04007.rst|lbl04007 - Using the Kill Bin]]


**  for who is the procedure relevant, typically the operator and anyone present while it's carried out, it's also worth mentioning the risks here and that the operator is responsible for their health and safety and that of anyone else present during the procedure.
== Definitions ==


'''4. Materials'''
; BSO
: Biological Safety Officer
; SOP
: Standard Operating Procedure


**  Are there any extra materials required, in the case of operating an instrument, peice of equipment or in a molecular biology procedure this might be consumables, reagents, chemicals or other instrumentation, e.g pipets. Any special PPE can be mentioned here.
== Procedures ==


'''5. Related documents'''
=== Daily Maintenance and Cleaning procedures ===


**  Are other SOPs (or other documents within our framework) related to this procedure. If so then listing them here can be useful, for example the media prep SOP might be relevant when carrying out the transformation procedures and it saves time to link it here rather than describe the preparation of media in every procedure where it is used (bit like an include/import/use lol).
==== Before starting work ====


'''6. Definitions'''
# At the beginning of daily sessions the worktops should be cleared and any washing done and cleaned items returned to the shelves.
# Bins should be emptied.
# Any unlabeled liquid media should be emptied into the kill bin.
# Unknown/unlabeled and dirty glassware left out should be autoclaved, washed and returned to shelves.
# Work areas should be disinfected with Surgical spirit before work commences.


**  Any useful glossary stuff
==== After Completing work ====
e.g:
SOP = Standard Operating Procedure


'''7. Procedures'''
# Clear the work surfaces of equipment and consumables. Don't leave things out.
# All media and cultures should be labeled.
# The Kill bin should be emptied of solid materials such as petri dishes. see [[lbl04007.rst|Emptying the kill bin]]
# Empty the geneal waste bins to the outside non-recyclables bin.
# Do the washing up.
# Make a record of the procedures and protocols in the lab day book.
# Ensure that electrical equipment is turned off, such as autoclave and RO pump


** 7.1  The actual steps of the procedure go here ...
=== Weekly cleaning and maintenance procedures ===
        7.1.1 They can be embeded/indented as much as is reasonably helpful to describe the procedure step by step
** 7.2 It should be written in such a way that it would help anyone new to the lab to perform the procedure without having prior expertise.


'''8. Resources'''
# Mop the floor with a 10% bleach solution
# Refill the Bleach solution in the kill bin. See section [[lbl04007.rst|Refuilling the kill bin]]
# Stock check the levels of consumables
#* Nitrile gloves
#* The spill kit
#* Bleach
#* Sterilizing Spray


** This is where eternal useful documention can be linked, much like the references section in any academic paper.
=== Ah-Hoc cleaning and maintenance procedures ===


==== Disposal of equipment ====


...
Equipment that is disposed of, due to obsoletion or damage etc, will need to be cleaned and decontaminated if it has come into contact with GMO. This procdure must be assessed and carried out on a case by case basis.


Please contact the BSO if you plan to remove any equipment from the biolab.


== Resources ==


[[Category:Biohacking]]
The SACGM Compendium of guidance Part 3: Containment and control of activities involving genetically modified microorganisms<br />http://www.hse.gov.uk/biosafety/gmo/acgm/acgmcomp/part3.pdf
[[Category:SOP]]

Latest revision as of 22:15, 26 January 2015

LBL08001 - Maintenance and Cleaning

Author: Tom Hodder Approved by: S. Thompson SOP No. LBL08001
Signed: Signed: Effective from:
Date: Date: Last edited:

Purpose

Maintenance and Cleaning are fundemental to the operation of a safe and successful biolab. This document provides the daily, weekly and ad-hoc procedures that are in place.

Scope

  • This SOP applies to all users in all circumstances in the LBL biolab without exception.
  • In the case of specific instructions for Maintenance and Cleaning of equipment the user should defer to the relevant SOP for that particular piece of equipment.

Responsibilities

  • All users of the biolab are responsible for ensuring the clean and safe operation of equipment, lab furniture and tools.
  • The Biological Safety Officer has oversight and responsilibity to make final decisions regarding maintenance and cleaning.

There are risks associated with certain cleaning products. Bleach is considered a hazardous substance and care must be taken to follow the instructions on the label.

It is the user's responsibility to check the MSDS for any chemical or cleaning products they are using.

Materials

The following items should be available in the lab

  • Disposable Nitrile gloves in various sizes
  • Plastic aprons
  • Eye protection
  • Plastic over-shoe protection
  • Cleaning products are available under the sink
  • A Mop and bucket
  • Blue roll
  • Disinfectant
  • Sterilzing Spray

Related documents

lbl08003 - Handling spillage of micro-organisms
lbl04007 - Using the Kill Bin

Definitions

BSO
Biological Safety Officer
SOP
Standard Operating Procedure

Procedures

Daily Maintenance and Cleaning procedures

Before starting work

  1. At the beginning of daily sessions the worktops should be cleared and any washing done and cleaned items returned to the shelves.
  2. Bins should be emptied.
  3. Any unlabeled liquid media should be emptied into the kill bin.
  4. Unknown/unlabeled and dirty glassware left out should be autoclaved, washed and returned to shelves.
  5. Work areas should be disinfected with Surgical spirit before work commences.

After Completing work

  1. Clear the work surfaces of equipment and consumables. Don't leave things out.
  2. All media and cultures should be labeled.
  3. The Kill bin should be emptied of solid materials such as petri dishes. see Emptying the kill bin
  4. Empty the geneal waste bins to the outside non-recyclables bin.
  5. Do the washing up.
  6. Make a record of the procedures and protocols in the lab day book.
  7. Ensure that electrical equipment is turned off, such as autoclave and RO pump

Weekly cleaning and maintenance procedures

  1. Mop the floor with a 10% bleach solution
  2. Refill the Bleach solution in the kill bin. See section Refuilling the kill bin
  3. Stock check the levels of consumables
    • Nitrile gloves
    • The spill kit
    • Bleach
    • Sterilizing Spray

Ah-Hoc cleaning and maintenance procedures

Disposal of equipment

Equipment that is disposed of, due to obsoletion or damage etc, will need to be cleaned and decontaminated if it has come into contact with GMO. This procdure must be assessed and carried out on a case by case basis.

Please contact the BSO if you plan to remove any equipment from the biolab.

Resources

The SACGM Compendium of guidance Part 3: Containment and control of activities involving genetically modified microorganisms
http://www.hse.gov.uk/biosafety/gmo/acgm/acgmcomp/part3.pdf

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