E. coli GFP transformation

Genetic modification

We're interested in doing genetic modification on micro-organisms at the London Hackspace. Ideas for stuff to do:

E coli transformed with PUC19 using ampicillin selection

28 mar 15

• 2ml ecoli culture (bottle on shelf) incubated in 200ml LB (bottle in fridge)
• -ve control is untransformed ecoli from same culture put on LBA
• +ve control is transformed ecoli from same culture put on LB plates
• Transform ecoli with PUC19 using electroporation
• Plate onto LBA.

GFP transformation

• The 'hello world' GFP experiment. Biorad do a kit. Carolina do just the plasmid

Growing competent E. coli

Here is the protocol we used with UCL to grow competent cells during the making of the public biobrick. So we need to get E. coli (one possibility. Could also try NCBE. They sell e. coli as a transformer kit replacement part. Finally these are suggested for schools by NCBE: Sciento, Blades Biological and Philip Harris Education), LB - £66, 0.1M CaCl2 - £32.50, M9 salts - £57.60, MgSO4 - £20, bacteriological agar solution - £42.90, thiamine - ~£20, D glucose - ~£20, and plates. All links are only the first ones I found, maybe cheaper options available.