283
edits
LB Medium: Difference between revisions
From London Hackspace Wiki
no edit summary
(Created page with "== Info == Used for growing ''E. coli''. == Protocol == # Using a balance weigh out the following and transfer to a '''1 litre''' screwcap bottle: #* '''2...") |
No edit summary |
||
| Line 6: | Line 6: | ||
# Using a balance weigh out the following and transfer to a '''1 litre''' screwcap bottle: | # Using a balance weigh out the following and transfer to a '''1 litre''' screwcap bottle: | ||
#* '''25g LB powder''' | #* '''25g LB-Miller powder''' *or* '''20g LB-Lennox powder''' *or* '''15.5g LB-Luria powder''' | ||
#* (15g agar if making solid medium for plates) | #* (15g agar if making solid medium for plates) | ||
# Half fill the bottle with water | # Half fill the bottle with water | ||
| Line 17: | Line 17: | ||
If adding antibiotic, add after autoclaving. | If adding antibiotic, add after autoclaving. | ||
25g of LB powder consists of: | 25g of LB-Miller powder consists of: | ||
* 10g tryptone | |||
* 5g yeast extract | |||
* 10g NaCl | |||
20g of LB-Lennox powder consists of: | |||
* 10g tryptone | |||
* 5g yeast extract | |||
* 5g NaCl | |||
15.5g of LB-Luria powder consists of: | |||
* 10g tryptone | |||
* 5g yeast extract | |||
* 0.5g NaCl | |||
