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PCR: Difference between revisions
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→Colony PCR
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= Colony PCR = | = Colony PCR = | ||
* | * Take colony, mix with 500ul of RO water | ||
* Boiling lysis program | |||
* Then run PCR using [[PCR_Machine| Techne TC-312]] | |||
* M13 primers (LacZ region of PUC19) and S16 primers (universal bacteria) | |||
* COL program. 30 cycles. Annealing is 53C (ideal for S16, 56 would be better for M16). Hold at 4 | |||
=== PCR primer design === | === PCR primer design === | ||
