E. coli transformation
Revision as of 18:25, 2 April 2015 by Mycoplasma (talk | contribs) (Created page with "= E coli transformed with PUC19 using ampicillin selection and electroporation = See [here](https://docs.google.com/document/d/1x5DkcQTWwBIKxbLGHKAjH2Sw1wdQL2B12tLCeF21b1c/ed...")
E coli transformed with PUC19 using ampicillin selection and electroporation
See [here](https://docs.google.com/document/d/1x5DkcQTWwBIKxbLGHKAjH2Sw1wdQL2B12tLCeF21b1c/edit?usp=sharing) for protocol
28 mar 15
- 2ml ecoli culture (bottle on shelf) incubated in 200ml LB (bottle in fridge)
- -ve control is untransformed ecoli from same culture put on LBA
- +ve control is transformed ecoli from same culture put on LB plates
- Transform ecoli with PUC19 using electroporation
- Plate onto LBA.
- No e coli survived on plates, whether with Amp or not. Could be various reasons.
GFP transformation
Growing chemically competent E. coli
Here is the protocol we used with UCL to grow competent cells during the making of the public biobrick. So we need to get E. coli (one possibility. Could also try NCBE. They sell e. coli as a transformer kit replacement part. Finally these are suggested for schools by NCBE: Sciento, Blades Biological and Philip Harris Education), LB - £66, 0.1M CaCl2 - £32.50, M9 salts - £57.60, MgSO4 - £20, bacteriological agar solution - £42.90, thiamine - ~£20, D glucose - ~£20, and plates. All links are only the first ones I found, maybe cheaper options available.