DNA extraction using Chelex 100
Revision as of 00:28, 24 August 2012 by Mycoplasma (talk | contribs)
- Fill half a tube (500ul) with 5% chelex 100 (try and get 50% beads and 50% clear liquid)
- Scrape off some cheek cells into the end of a pipette tip, add this and some saliva to the tube and mix
- Cover with a drop of paraffin Incubate at 56°C for 30 mins, then at 94°C for 10 mins
- Centrifuge briefly and take supernatant
- Store at -20°C