Difference between revisions of "E. coli GFP transformation"
(Created page with "= Genetic modification = We're interested in doing genetic modification on micro-organisms at the London Hackspace. == Legal == There are a number of things we have to do in o...") |
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= Genetic modification = | = Genetic modification = | ||
| − | We're interested in doing genetic modification on micro-organisms at the London Hackspace. | + | We're interested in doing genetic modification on micro-organisms at the London Hackspace. Checklist for GFP experiement: |
| + | |||
| + | Freezer. | ||
| + | -20C or colder. | ||
| + | Temperature logger? | ||
| + | Fridge? | ||
| + | Autoclave. | ||
| + | Pressure cooker, electric hob and autoclave tape? | ||
| + | Incubator. | ||
| + | Build or buy. | ||
| + | Cleaning: | ||
| + | Bleach, gloves, paper towels, hand cleaning gel. | ||
| + | Bunsen burner + fuel. | ||
| + | Glassware, pipettes, tools. | ||
| + | Ethanol. | ||
| + | Lots. | ||
| + | |||
| + | To apply for a license we will probably first need: | ||
| + | Written protocols for stuff, especially cleaning up. | ||
| + | |||
| + | Disposal. | ||
| + | Chemical. | ||
| + | Sterilisation. | ||
| + | Do we need incineration? Find a waste disposal company that will take biohazard material? | ||
| + | |||
| + | Restriction enzymes. | ||
| + | At least two. | ||
| + | Pricey? | ||
| + | GFP plasmid. | ||
| + | Antibiotics. | ||
| + | Ampicillin. | ||
| + | Initial e. coli culture. | ||
| + | Growth medium/ broth. | ||
| + | LB. | ||
| + | Plates. | ||
| + | Standard 10cm plates. | ||
| + | Agarose. | ||
| + | Ice. | ||
| + | 40C bath. | ||
| + | |||
| + | Proper storage. | ||
| + | |||
| + | Centrifuge | ||
| + | - bigger : hold | ||
| + | |||
| + | Miniprep kit | ||
| + | |||
| + | Maxiprep kit | ||
== Legal == | == Legal == | ||
Revision as of 22:31, 21 July 2012
Genetic modification
We're interested in doing genetic modification on micro-organisms at the London Hackspace. Checklist for GFP experiement:
Freezer. -20C or colder. Temperature logger? Fridge? Autoclave. Pressure cooker, electric hob and autoclave tape? Incubator. Build or buy. Cleaning: Bleach, gloves, paper towels, hand cleaning gel. Bunsen burner + fuel. Glassware, pipettes, tools. Ethanol. Lots.
To apply for a license we will probably first need: Written protocols for stuff, especially cleaning up.
Disposal. Chemical. Sterilisation. Do we need incineration? Find a waste disposal company that will take biohazard material?
Restriction enzymes. At least two. Pricey? GFP plasmid. Antibiotics. Ampicillin. Initial e. coli culture. Growth medium/ broth. LB. Plates. Standard 10cm plates. Agarose. Ice. 40C bath.
Proper storage.
Centrifuge - bigger : hold
Miniprep kit
Maxiprep kit
Legal
There are a number of things we have to do in order to set up a GM lab.
The relevant regulations are The Genetically Modified Organisms (Contained Use) Regulations 2000 (PDF)
Here is a summary of those regulations: GMO Regulations 2000 summary