Difference between revisions of "E. coli GFP transformation"
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= Genetic modification = | = Genetic modification = | ||
| − | We're interested in doing genetic modification on micro-organisms at the London Hackspace. | + | We're interested in doing genetic modification on micro-organisms at the London Hackspace. Needed for GFP experiment (that we don't already have): |
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Fridge? | Fridge? | ||
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Incubator. | Incubator. | ||
Build or buy. | Build or buy. | ||
Revision as of 22:32, 21 July 2012
Genetic modification
We're interested in doing genetic modification on micro-organisms at the London Hackspace. Needed for GFP experiment (that we don't already have):
Fridge? Incubator. Build or buy. Cleaning: Bleach, gloves, paper towels, hand cleaning gel. Bunsen burner + fuel. Glassware, pipettes, tools. Ethanol. Lots.
To apply for a license we will probably first need: Written protocols for stuff, especially cleaning up.
Disposal. Chemical. Sterilisation. Do we need incineration? Find a waste disposal company that will take biohazard material?
Restriction enzymes. At least two. Pricey? GFP plasmid. Antibiotics. Ampicillin. Initial e. coli culture. Growth medium/ broth. LB. Plates. Standard 10cm plates. Agarose. Ice. 40C bath.
Proper storage.
Centrifuge - bigger : hold
Miniprep kit
Maxiprep kit
Legal
There are a number of things we have to do in order to set up a GM lab.
The relevant regulations are The Genetically Modified Organisms (Contained Use) Regulations 2000 (PDF)
Here is a summary of those regulations: GMO Regulations 2000 summary