Difference between revisions of "Juiceyprint Proposal"
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==previous similar work== | ==previous similar work== | ||
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| + | <pre> | ||
| + | @article{levskaya2005synthetic, | ||
| + | title={Synthetic biology: engineering Escherichia coli to see light}, | ||
| + | author={Levskaya, Anselm and Chevalier, Aaron A and Tabor, Jeffrey J and Simpson, Zachary Booth and Lavery, Laura A and Levy, Matthew and Davidson, Eric A and Scouras, Alexander and Ellington, Andrew D and Marcotte, Edward M and others}, | ||
| + | journal={Nature}, | ||
| + | volume={438}, | ||
| + | number={7067}, | ||
| + | pages={441--442}, | ||
| + | year={2005}, | ||
| + | publisher={Nature Publishing Group} | ||
| + | } | ||
| + | </pre> | ||
| + | [http://lists.kentgeek.org/pipermail/lhs-biohacking/attachments/20140126/71aa6ce7/attachment-0002.pdf PDF] | ||
| + | |||
| + | http://jb.asm.org/content/195/22/5072 | ||
| + | |||
| + | [http://lists.kentgeek.org/pipermail/lhs-biohacking/attachments/20140126/71aa6ce7/attachment-0003.pdf PDF] | ||
==Working hypothesis and questions== | ==Working hypothesis and questions== | ||
Revision as of 21:11, 12 March 2014
London Biohackers DRAFT IGEM Research Proposal "JuiceyPrint" (working title)
Background and motivation
Layering of macromolecular substrates has many potential applications in biotechnology, biomedical science and other technology projects and as such there is a strong motivation for constructing sheets of biopolymers including cellulose, collagen and silk with micron scale architecture. Inspiration here is drawn from lithographic methods where differential light exposure on a receptive surface can be used to control the deposition or removal of a substance of choice. In this case it is intended to be mediated by a strain of light sensitive bacterium whose metabolic production of a selected structural macromolecule can be regulated through its coupling to a light sensitive signal transduction pathway.
<diagram>
previous similar work
@article{levskaya2005synthetic,
title={Synthetic biology: engineering Escherichia coli to see light},
author={Levskaya, Anselm and Chevalier, Aaron A and Tabor, Jeffrey J and Simpson, Zachary Booth and Lavery, Laura A and Levy, Matthew and Davidson, Eric A and Scouras, Alexander and Ellington, Andrew D and Marcotte, Edward M and others},
journal={Nature},
volume={438},
number={7067},
pages={441--442},
year={2005},
publisher={Nature Publishing Group}
}
http://jb.asm.org/content/195/22/5072
Working hypothesis and questions
how we expect things to work, potential shortcomings and how to get round them, specific problems/outcomes we hope to address
Experimental design and methodology
details of genes, components/biobricks etc and procedures.
Future prospects
if this works what might it lead to, what can we look at next or refine