Difference between revisions of "LBL05001"

From London Hackspace Wiki
Jump to navigation Jump to search
(Created page with "== Containment Level One For BioLab Users == {| class="wikitable" |Author: |Approved by: S. Thompson |SOP No. LBL05001 |- |Signed: |Signed: |Effective from: |- |Date...")
 
Line 29: Line 29:
 
'''1. Purpose'''
 
'''1. Purpose'''
  
−
**  general description of the SOP purpose
+
Generally, under the ''Genetically Modified Organisms (Contained Use) Regulations 2014'' any CL1 project being undertaken in the biolab must follow the procedures outlined in this document in conjunction with any procedures specified in the risk assessment for that project.
  
 
'''2. Scope'''
 
'''2. Scope'''
  
−
**  the extent of the SOP in terms of to what equipment and procedures it applies, also when and where (which will always be at all times in the LBL lab).
+
This relates to CL1 projects.
  
 
'''3. Responsibilities'''
 
'''3. Responsibilities'''
  
−
**  for who is the procedure relevant, typically the operator and anyone present while it's carried out, it's also worth mentioning the risks here and that the operator is responsible for their health and safety and that of anyone else present during the procedure.
+
This applies to anyone carrying out these procedures as part of a CL1 project. The user should ensure that others do not inadvertently compromise the containment of GMOs.
  
 
'''4. Materials'''
 
'''4. Materials'''
Line 45: Line 45:
 
'''5. Related documents'''
 
'''5. Related documents'''
  
−
**  Are other SOPs (or other documents within our framework) related to this procedure. If so then listing them here can be useful, for example the media prep SOP might be relevant when carrying out the transformation procedures and it saves time to link it here rather than describe the preparation of media in every procedure where it is used (bit like an include/import/use lol).
+
[[LBL06001]] Project Risk Assessment Procedure for Lab Users Proposing New Projects.
 +
[[LBL06002]] Risk Assessment Procedure for Lab Users Proposing Transport of GMOs Outside the Lab.
  
 
'''6. Definitions'''
 
'''6. Definitions'''
  
−
**  Any useful glossary stuff
+
GMO - Genetically Modified Organism
−
e.g:
+
GMM - Genetically Modified Micro-organism
−
SOP = Standard Operating Procedure
+
CL1 - Containment Level 1
  
 
'''7. Procedures'''
 
'''7. Procedures'''
  
−
** 7.1 The actual steps of the procedure go here ...
+
7.1 Risk Assessment
−
        7.1.1 They can be embeded/indented as much as is reasonably helpful to describe the procedure step by step
 
−
** 7.2 It should be written in such a way that it would help anyone new to the lab to perform the procedure without having prior expertise.
 
  
−
'''8. Resources'''
+
A written risk assessment must be approved  before work can commence on any CL1 project (see [[BioLab Usage Guidelines#Special GMO considerations]] for guidance on determining what is classed as CL1). The risk assessment procedure is detailed in [[LBL06001]] ''Project Risk Assessment Procedure for Lab Users Proposing New Projects''.
 +
 
 +
 
 +
7.2 Worksite
 +
 
 +
7.2.1 All work with GMOs as part of a CL1 project must take place within the BioLab. The BioLab is equipped with door access control in order to enable restriction of access to only trained users when appropriate. An observation window is also present.
 +
 
 +
7.2.2 All work with GMMs as part of a CL1 project must be carried out on the wet work workbench (white surface, on the left as one enters the lab). This bench is impervious to water, resistant to acids, alkalis, solvents, disinfectants and decontamination agents and easy to clean.
 +
 
 +
7.3 Protective clothing
 +
 
 +
Suitable protective clothing should be worn. The [[BioLab Usage Guidelines#Use of PPE]] gives guidance on this matter. If suitable PPE is not available, the BSO should be informed immediately before any practical work is commenced.
 +
 
 +
7.4 Inactivation of GMMs
 +
 
 +
All waste (e.g. disposable gloves, cultures of GMMs) produced during a CL1 project must be sterilised using the on-site autoclave according to the standard procedure detailed in [[LBL04006]] ''Using the Autoclave for Waste Deactivation''. The user should pay special attention to ensure the autoclave has undergone the daily test procedure.
  
−
** This is where eternal useful documention can be linked, much like the references section in any academic paper.
+
7.5 Safe storage of GMOs
  
 +
GMOs should be stored in an appropriate area of the BioLab in such a way as to minimise the risk of contamination. The BioLab is equipped with door access control in order to enable restriction of access to only trained users when appropriate.
  
−
...
+
'''8. Resources'''
  
 +
The Genetically Modified Organisms (Contained Use) Regulations 2014 Guidance on Regulations (http://www.hse.gov.uk/pubns/books/l29.htm)
  
  
 
[[Category:Biohacking]]
 
[[Category:Biohacking]]

Revision as of 03:21, 12 January 2015

Containment Level One For BioLab Users

Author: Approved by: S. Thompson SOP No. LBL05001
Signed: Signed: Effective from:
Date: Date: Last edited:

1. Purpose

Generally, under the Genetically Modified Organisms (Contained Use) Regulations 2014 any CL1 project being undertaken in the biolab must follow the procedures outlined in this document in conjunction with any procedures specified in the risk assessment for that project.

2. Scope

This relates to CL1 projects.

3. Responsibilities

This applies to anyone carrying out these procedures as part of a CL1 project. The user should ensure that others do not inadvertently compromise the containment of GMOs.

4. Materials

    • Are there any extra materials required, in the case of operating an instrument, peice of equipment or in a molecular biology procedure this might be consumables, reagents, chemicals or other instrumentation, e.g pipets. Any special PPE can be mentioned here.

5. Related documents

LBL06001 Project Risk Assessment Procedure for Lab Users Proposing New Projects. LBL06002 Risk Assessment Procedure for Lab Users Proposing Transport of GMOs Outside the Lab.

6. Definitions

GMO - Genetically Modified Organism GMM - Genetically Modified Micro-organism CL1 - Containment Level 1

7. Procedures

7.1 Risk Assessment

A written risk assessment must be approved before work can commence on any CL1 project (see BioLab Usage Guidelines#Special GMO considerations for guidance on determining what is classed as CL1). The risk assessment procedure is detailed in LBL06001 Project Risk Assessment Procedure for Lab Users Proposing New Projects.


7.2 Worksite

7.2.1 All work with GMOs as part of a CL1 project must take place within the BioLab. The BioLab is equipped with door access control in order to enable restriction of access to only trained users when appropriate. An observation window is also present.

7.2.2 All work with GMMs as part of a CL1 project must be carried out on the wet work workbench (white surface, on the left as one enters the lab). This bench is impervious to water, resistant to acids, alkalis, solvents, disinfectants and decontamination agents and easy to clean.

7.3 Protective clothing

Suitable protective clothing should be worn. The BioLab Usage Guidelines#Use of PPE gives guidance on this matter. If suitable PPE is not available, the BSO should be informed immediately before any practical work is commenced.

7.4 Inactivation of GMMs

All waste (e.g. disposable gloves, cultures of GMMs) produced during a CL1 project must be sterilised using the on-site autoclave according to the standard procedure detailed in LBL04006 Using the Autoclave for Waste Deactivation. The user should pay special attention to ensure the autoclave has undergone the daily test procedure.

7.5 Safe storage of GMOs

GMOs should be stored in an appropriate area of the BioLab in such a way as to minimise the risk of contamination. The BioLab is equipped with door access control in order to enable restriction of access to only trained users when appropriate.

8. Resources

The Genetically Modified Organisms (Contained Use) Regulations 2014 Guidance on Regulations (http://www.hse.gov.uk/pubns/books/l29.htm)

Retrieved from ‘https://wiki.london.hackspace.org.uk/w/index.php?title=LBL05001&oldid=44940’